实战经验！盲肠结扎穿孔术构建小鼠脓毒症模型_文献解读_Elabscie…
2024年3月20日 · 盲肠结扎穿孔（Cecal ligation and puncture, CLP）模型是最接近于人类脓毒症机制的模型，被称为脓毒症模型的“金标准”。 该模型通过结扎并穿孔盲肠，使混有多种细菌的肠内容物持续溢入腹腔，从而模拟细菌性腹膜炎的临床特点。
Sepsis modeling in mice: ligation length is a major severity …
2016年7月18日 · Our study suggests that the length of cecal ligation is a major severity factor in the mouse CLP model when needle size and the number of punctures are controlled. Furthermore, it underlines differences in the inflammatory response between rats and mice.
Cecal ligation puncture procedure - PubMed
2011年5月7日 · The most widely used clinical model is cecal ligation and puncture (CLP). The CLP model consists of the perforation of the cecum allowing the release of fecal material into the peritoneal cavity to generate an exacerbated immune …
Mouse Models of Sepsis - PubMed
Basic Protocol 1 details a non-synchronous cecal ligation and puncture (CLP) model of sepsis, where mice are subjected to polymicrobial exposure through surgery at different time points within 2 weeks.
Two murine models of sepsis: immunopathological differences …
2023年10月24日 · Endotoxic shock (ExSh) and cecal ligature and puncture (CLP) are models that induce sepsis. In this work, we investigated early immunologic and histopathologic changes induced by ExSh or CLP models in female and male mice.
小鼠脓毒症模型的建立和评价-【维普期刊官网】- 中文期刊服务平台
方法采用盲肠结扎穿孔法 (cecal ligation and puncture,CLP)诱导小鼠脓毒症,通过动物生存、术后小鼠载菌量、血常... 展开更多 目的建立小鼠脓毒症模型并进行评价,为研究脓毒症致病机制和开发抗炎药物提供模型动物。 方法采用盲肠结扎穿孔法 (cecal ligation and puncture,CLP)诱导小鼠脓毒症,通过动物生存、术后小鼠载菌量、血常规和血生化指标、细胞因子水平、组织病理变化等方面对模型进行评价。 结果小鼠的死亡率与盲肠结扎部位密切相关,盲肠结扎50%小鼠12 d存活率 …
Immunodesign of experimental sepsis by cecal ligation and puncture
2008年12月11日 · The model of CLP is widely used and known to closely mimic the pathophysiology of septic human patients. Accordingly, polymicrobial sepsis is associated with an early hyperdynamic phase and a...
Multi-Organ Transcriptome Dynamics in a Mouse Model of Cecal …
2021年6月3日 · We used RNA sequencing to characterize transcriptional changes in the early phase of sepsis, at 6, 12, 24 hour time points in lung, kidney, liver, and heart tissues, in a cecal ligation and puncture (CLP)-induced polymicrobial sepsis murine model.
Cecal ligation and puncture: the gold standard model for …
2011年4月1日 · Polymicrobial sepsis induced by cecal ligation and puncture (CLP) is the most frequently used model because it closely resembles the progression and characteristics of human sepsis. Here we summarize the role of several immune components in the pathogenesis of sepsis induced by CLP.
Mouse Models of Sepsis - Kannan - 2024 - Current Protocols
2024年3月4日 · Cecal ligation and puncture (CLP) is one such model that was first established in mice in 1983 (Baker et al., 1983). The CLP model captures some of the most important hallmarks of human sepsis, specifically the inflammation, lymphopenia, and long-term immunosuppression, making it the most comprehensive model used today.