EGFP :: Fluorescent Protein Database
EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.
EGFP Sequence and Map - SnapGene
SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. The maps, notes, and annotations in the zip file on this page …
Enhanced Green Fluorescent Protein - an overview - ScienceDirect
Enhanced Green Fluorescent Protein (EGFP) is a genetically modified version of the Green Fluorescent Protein (GFP) that emits 35 times more fluorescence when excited with ultraviolet or blue light, making it much brighter.
Difference Between GFP and EGFP - Pediaa.Com
2018年6月12日 · The main difference between GFP and EGFP is that the GFP (stands for Green Fluorescent Protein) is a protein that exhibits bright green fluorescence when exposed to blue light whereas the EGFP (stands for Enhanced Green Fluorescence Protein) exhibits stronger fluorescence than GFP.
Crystal Structure of Enhanced Green Fluorescent Protein to 1.35 Å ...
Enhanced Green Fluorescent Protein (EGFP) is one of the most widely used engineered variants of the original wild-type Green Fluorescent Protein. Here, we report the high resolution (1.35 Å) structure of EGFP crystallised in its untagged sequence ...
Enhanced Green Fluorescent Protein - ScienceDirect Topics
Fluorescent protein–based reporter systems also became very popular during the 1990s, especially for in vitro and embryogenesis studies. For example, green fluorescent protein (GFP) has evolved from a little known protein to a common widely used tool in …
2Y0G - RCSB PDB
2010年12月2日 · Enhanced Green Fluorescent Protein (EGFP) is a variant of wild-type Green Fluorescent Protein from the jellyfish Aequorea victoria, whose mutations S65T and F64L increase brightness and folding efficiency.
The enhanced green fluorescent protein as a tool for the ... - PubMed
From these experiments EGFP emerges as an ideal molecule for quantitative studies of intra and intercellular tagged-protein dynamics and fluorescence-activated cell sorting, but not for monitoring single-protein trafficking over extended periods of time.
Design and Use of Fluorescent Fusion Proteins in Cell Biology
Unmodified fluorescent proteins (FPs) can be visualized by fluorescence microscopy and can serve as probes of environments within living cells. The addition of targeting and retention sequences to FPs can be exploited to highlight specific …
Fluorescent proteins such as eGFP lead to catalytic oxidative stress …
Enhanced GFP (eGFP) was optimized from wild-type GFP for increased fluorescence yield and improved expression in mammalian systems [3]. Many GFP-like fluorescent proteins have been discovered, optimized or created, such as the red fluorescent protein TagRFP [4].
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