Gene cassette - Wikipedia
In genetic engineering, a gene cassette is a manipulable fragment of DNA carrying, and capable of expressing, one or more genes of interest between one or more sets of restriction sites. It can be transferred from one DNA sequence (usually on a vector ) to another by 'cutting' the fragment out using restriction enzymes and 'pasting' it back ...
Gene Cassette - an overview | ScienceDirect Topics
Gene cassettes are small segments of DNA, frequently found inserted into an integron or in a free circular structure (Delihas, 2011; Partridge et al., 2018). Gene cassettes usually consist of one or two genes, regularly lacking a recombination site (attC) and a promoter (Partridge et al., 2018).
Gene Cassette - an overview | ScienceDirect Topics
Gene cassettes are short strands of DNA usually found integrated into integrons or in free circular structures. Gene cassettes typically contain one or two genes with no recombination site (attC) or promoter [52]. A few components, including an intI gene, a promoter and an attI recombination site, can be used to identify integrons [53 ...
Mobile gene cassettes and integrons: capture and spread of genes …
An integron is a genetic unit that includes the determinants of the components of a site-specific recombination system capable of capturing and mobilizing genes that are contained in mobile elements called gene cassettes. An integron also provides a promoter for expression of the cassette genes, and …
Integron cassettes integrate into bacterial genomes via ... - Nature
2024年1月3日 · Integrons are genetic elements involved in bacterial adaptation which capture, shuffle and express genes encoding adaptive functions embedded in cassettes. These events are governed by the...
Gene cassettes and cassette arrays in mobile resistance integrons
An updated compilation of gene cassettes found in sequences of such 'mobile resistance integrons' in GenBank was facilitated by a specially developed automated annotation system. At least 130 different (<98% identical) cassettes that carry known or predicted antibiotic resistance genes were identified, along with many cassettes of unknown function.
Integrons and gene cassettes: a genetic construction kit for …
1999年1月1日 · Gene cassettes are discrete genetic elements that may exist as free, circular, non-replicating DNA molecules when moving from one genetic site to another, 3 but which are normally found as linear sequences that constitute part of a larger DNA molecule, such as a plasmid or bacterial chromosome.
Predicting the taxonomic and environmental sources of integron gene …
2021年8月9日 · Integrons are bacterial genetic elements that can capture mobile gene cassettes. They are mostly known for their role in the spread of antibiotic resistance cassettes, contributing...
Identification and analysis of integrons and cassette arrays in ...
We identified such genes in 105 cassettes, i.e., in 3% of all cassettes from complete integrons (3116 cassettes). Most resistance cassettes were found in class 1 to 5 integrons (90% of them), even if the latter contained only 4.5% of all cassettes.
Gene Cassette - an overview | ScienceDirect Topics
A gene cassette refers to a compact organization of genetic material that typically includes a single gene or open reading frame, along with a recombination site. It can exist in either a linear or circular form and is commonly found integrated at a specific site in an integron.
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