Overview: generation of gene knockout mice - PubMed
1973年12月24日 · The technique of gene targeting allows for the introduction of engineered genetic mutations into a mouse at a determined genomic locus. The process of generating mouse models with targeted mutations was developed through both the discovery of homologous recombination and the isolation of murine embr …
One-step generation of complete gene knockout mice and …
Here we show that single gene or multiple genes can be completely knocked out in mouse and monkey embryos by zygotic injection of Cas9 mRNA and multiple adjacent single-guide RNAs (spaced 10-200 bp apart) that target only a single key exon of each gene.
The Construction of Transgenic and Gene Knockout/Knockin Mouse …
Although some physiological and immunological differences exist between mice and humans (Mestas 2004), genomic sequencing has demonstrated that of ~30,000 genes in both mice and humans only 300 (i.e., 1%) are unique to either species (Waterston 2002).
Pipeline for the generation of gene knockout mice using dual …
2019年3月1日 · Here, we provide a precise protocol for generation of a knockout mouse model for β-thalassemia by targeted deletion of exons 2 and 3 in mouse Hbb-bs gene using CRISPR/Cas9.
Genome-wide Generation and Systematic Phenotyping of Knockout Mice ...
2013年7月18日 · Mutations in whole organisms are powerful ways of interrogating gene function in a realistic context. We describe a program, the Sanger Institute Mouse Genetics Project, that provides a step toward the aim of knocking out all genes and screening each line …
Overview: Generation of Gene Knockout Mice - Hall - 2009
2009年9月1日 · Abstract The technique of gene targeting allows for the introduction of engineered genetic mutations into a mouse at a determined genomic locus. The process of generating mouse models with targeted...
Overview: generation of gene knockout mice. - Europe PMC
2009年9月1日 · To date, about 11,000 genes have been knocked out in mice, which accounts for roughly half of the mouse genome ( Vogel, 2007; Sikorski and Peters, 1997 ). Through a combination of gene targeting and gene trapping, a global effort is underway to make a knockout mouse for all of the 25,000 mouse genes ( Grimm, 2006 ).
Overview: Generation of Gene Knockout Mice - Semantic Scholar
2009年9月1日 · This review aims to provide a general overview of the Cre/loxP system, elaborate on the different keratinocyte specific knockout mice used to study gene function in keratinocytes, and finally demonstrate the generation of a conditional knockout mice.
Generation of Conditional Knockout Mice | SpringerLink
2014年1月1日 · Conditional knockout mouse models are powerful tools to examine the biological and molecular function (s) of genes in specific tissues. The general procedure to generate such genetically engineered mouse models consists of three main steps.
Efficient generation of conditional knockout mice via sequential ...
2017年8月11日 · To study the gene functions in adult mice, conditional knockout, which allows for precise control of genetic modifications in specific tissues and at specific stages, is necessary. The most...