Mechanism underlying potato spindle tuber viroid affecting tomato …
2021年3月25日 · Severe infection of the tomato cultivar ‘Rutgers’ with potato spindle tuber viroid (PSTVd) variants resulted in systemic stunting, leaf malformation, and vein necrosis; accompanied by a rapid increase in reactive oxygen species (ROS) and abnormally high expression of the stress-responsive microRNAs, miR398 and miR398a-3p.
Xanthohumol and echinocystic acid induces PSTVd tolerance in tomato
2024年6月20日 · In this study, we assessed PSTVd tolerance in semi-cultivated tomatoes based on morphological traits, physiological changes, and the accumulation of replicons; the possible viroid-tolerance-inducing metabolites of the PSTVd-resistant tomato genotype were identified by non-target metabonomics analysis.
Effects of a Potato Spindle Tuber Viroid Tomato Strain on the …
2019年9月25日 · The aims of this study were to determine the pathogenicity of a PSTVd strain from tomato by infecting (i) the classical PSTVd indicator hosts tomato Rutgers and potato Russet Burbank and (ii) representatives of newer commercial cultivars currently grown widely in Australia and elsewhere: cultivars Atlantic (potato), Nadine (potato), Petula ...
Potato spindle tuber viroid - PMC - National Center for …
5 天之前 · Here we review recent literature data concerning the genetics of Potato spindle tuber viroid (PSTVd) and the mechanisms related to its pathological effect on the host plants. PSTVd can be transmitted vertically through microspores and macrospores, but not with pollen from another infected plant.
Potato Spindle Tuber Viroid Modulates Its Replication through a …
2018年10月10日 · As circular noncoding RNAs, viroids can replicate and spread in plants, often resulting in disease symptoms. Potato spindle tuber viroid (PSTVd), the type species of nuclear-replicating viroids, requires a unique splicing form of transcription factor IIIA …
Knockout of SlDCL2b attenuates the resistance of tomato to …
2024年3月10日 · Here, we determined the function of tomato DCL2b (SlDCL2b) in defence responses against potato spindle tuber viroid (PSTVd) infection using SlDCL2b loss-of-function tomato mutant plants. Compared with wild-type plants, mutant plants were more susceptible to PSTVd infection, developing more severe symptoms earlier and accumulating higher levels ...
Predicting symptom severity in PSTVd‐infected tomato plants …
2024年7月2日 · For the inoculation experiment, we randomly selected 33 out of the 307 PSTVd isolates to assess their symptom severity on tomato plants (S. lycopersicum ‘Rutgers’). Infectious transcripts of the PSTVd isolates were prepared via in vitro transcription using the T7 RiboMax Large Scale RNA kit (Promega), following the manufacturer's instructions.
Effects of potato spindle tuber viroid infection on tomato …
2016年8月20日 · The cDNA of PSTVd-intermediate variant was used to inoculate 3–4 true leaves twenty days old, greenhouse grown tomato seedlings of Moneymaker, and buffer alone inoculated plants served as control. Symptoms of tomato plants infected by PSTVd included epinasty, stunting, and leaf rugosity and distortion as has previously described by Diener (1987).
Frontiers | Insights Into Potato Spindle Tuber Viroid Quasi-Species ...
2020年7月2日 · In this study, the ten most abundant sequence variants of potato spindle tuber viroid RG1 (PSTVd) expressed at different time intervals in PSTVd infected tomato plants were identified by high-throughput sequencing.
Predicting symptom severity in PSTVd‐infected tomato plants …
2024年7月2日 · Here, we developed an algorithm using unsupervised machine learning to predict the severity of disease symptoms caused by viroids (e.g., potato spindle tuber viroid; PSTVd) in host plants (e.g., tomato).