CRISPR Design Tool - Horizon Discovery
Horizon’s Edit-R CRISPR design tool generates guide RNAs for straightforward knockout experiments, as well as custom site-specific guides that target user-defined regions of a gene.
CRISPR: Guide to gRNA design - SnapGene
CRISPR: Guide to gRNA design. Learn the steps involved in CRISPR ranging from designing your gRNA and repair templates to approaches to verify your genome edit.
E-CRISP Design
Enter 1 to find designs which target the first exon exon. Or enter the word any to find designs that hit any exon. Check this box to assess restricion enzyme cut sites in the whole target sequence. Choose between different pre-defined restriction enzyme subsets.
GitHub - crisprVerse/crisprDesign: Comprehensive design of CRISPR …
2022年7月1日 · crisprDesign is the core package of the crisprVerse ecosystem, and plays the role of a one-stop shop for designing and annotating CRISPR guide RNA (gRNA) sequences.
WGE - CRISPR design tool - Wellcome Sanger Institute
WGE is a highly interactive, web-based visual tool that employs an embedded genome browser and database to assist scientists in designing genome editing strategies using the CRISPR/Cas9 system. Genome editing is the science of altering specific portions of DNA so that it behaves in a subtly different way within a cell.
Design and analysis of CRISPR–Cas experiments - Nature
2020年4月13日 · Here we review three categories of software tools for three experimental approaches: the design and selection of guide RNAs, focused analysis of the results of CRISPR editing and knockout...
Genome engineering using the CRISPR-Cas9 system - Nature
2013年10月24日 · The CRISPR Design Tool provides the sequences for all oligos and primers necessary for (i) preparing the sgRNA constructs, (ii) assaying target modification efficiency and (iii)...
How to Design Your gRNA for CRISPR Genome Editing - Addgene
2017年5月3日 · CRISPR technology has made it easier than ever both to engineer specific DNA edits and to perform functional screens to identify genes involved in a phenotype of interest. This blog post will discuss differences between these approaches, and provide updates on …
A comprehensive Bioconductor ecosystem for the design of CRISPR …
2022年11月2日 · Here, we present a new ecosystem of R packages, called crisprVerse, that enables efficient gRNA design and annotation for a multitude of CRISPR technologies. This includes CRISPR knockout...
DeepCRISPR : optimized CRISPR guide RNA design by deep …
2018年6月26日 · Here we present DeepCRISPR, a comprehensive computational platform to unify sgRNA on-target and off-target site prediction into one framework with deep learning, surpassing available state-of-the-art in silico tools.