Loop-mediated isothermal amplification - Wikipedia
Loop-mediated isothermal amplification (LAMP) is a single-tube technique for the amplification of DNA [2] for diagnostic purposes and a low-cost alternative to detect certain diseases. [3] LAMP is an isothermal nucleic acid amplification technique.
Loop-Mediated Isothermal Amplification - NEB
Loop-mediated isothermal amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA for a highly specific amplification reaction. A strand-displacing DNA polymerase initiates synthesis and 2 specially designed primers form “loop” structures to facilitate subsequent rounds of amplification through extension on the ...
Loop-mediated isothermal amplification of DNA - PubMed
2000年6月15日 · We have developed a novel method, termed loop-mediated isothermal amplification (LAMP), that amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six disti …
Loop-Mediated Isothermal Amplification - ScienceDirect
Loop-mediated isothermal amplification (LAMP) is a novel molecular technique increasingly employed for malaria diagnosis. In this method, target DNA is amplified using specific primers along with Bst polymerase at isothermal conditions (60–65 °C). The whole reaction takes around 1 h, and the DNA amplification process produces magnesium ...
LAMP - a method of isothermal DNA amplification - ResearchGate
2016年1月1日 · Here we show an amplification-coupled detection method for directly measuring released hydrogen ions during the loop mediated isothermal amplification (LAMP) procedure by using a pH meter.
Loop‐mediated isothermal amplification (LAMP): a versatile …
2017年11月22日 · Loop-mediated isothermal amplification (LAMP) amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions by using a DNA polymerase with high displacement strand activity and a set of specifically designed primers to …
Loop-Mediated Isothermal Amplification - ScienceDirect
Loop-mediated isothermal amplification (LAMP) is a powerful and isothermal technique that amplifies a few copies of target DNA with high specificity, efficiency, and rapidity at 65°C without the need for template denaturation, using a set of four specially designed primers and a DNA polymerase with strand displacement activity.
Loop-mediated isothermal amplification (LAMP) – review and ...
2020年1月9日 · Loop-mediated isothermal amplification (LAMP) is well known for its robust and highly sensitive and specific amplification of target DNA, which is achieved by utilizing up to six primers. Moreover, LAMP excels through its isothermal and energy efficient amplification requirements, rendering it a prime candidate for low-cost diagnostics and ...
Loop-mediated isothermal amplification (LAMP) of gene
2008年4月24日 · Loop-mediated isothermal amplification (LAMP) is a simple, rapid, specific and cost-effective nucleic acid amplification method when compared to PCR, nucleic acid...
Loop-mediated Isothermal Amplification - Bitesize Bio
2020年1月16日 · Loop-mediated Isothermal amplification (LAMP), is an emerging technology that allows DNA amplification at a constant temperature. The key to this principle is the use of a DNA polymerase that possesses strand displacement activity.