Xu CHEN | Doctor of Medicine | Shanghai Jiao Tong University, …
γδ T cells play a critical role in homeostasis and diseases such as infectious diseases and tumors in both mice and humans. They can be categorized into two main functional subsets:...
Expansion and Polarization of Human γδT17 Cells in vitro from ...
2024年1月5日 · Key Features • Expansion of γδ T cells from peripheral blood mononuclear cells. • Human IL-17A-producing γδ T-cell differentiation and expansion using IL-7 and anti-γδTCR. • Analysis of IL-17A production post γδ T-cell expansion. This protocol is used in: Science Advances (2022), DOI: 10.1126/sciadv.abm9120.
Expansion and Polarization of Human γδT17 Cells in vitro from ...
2024年1月1日 · In this protocol, we describe a method for in vitro expansion and polarization of human γδT17 cells from PBMCs. • Expansion of γδ T cells from peripheral blood mononuclear cells. • Human IL-17A-producing γδ T-cell differentiation and expansion using IL-7 and anti-γδTCR. • Analysis of IL-17A production post γδ T-cell expansion.
免疫细胞分化 -免疫学-BIO-PROTOCOL
2024年1月5日 · In this protocol, we describe a method for in vitro expansion and polarization of human γδT17 cells from PBMCs. Key Features. • Expansion of γδ T cells from peripheral blood mononuclear cells. • Human IL-17A-producing γδ T-cell differentiation and expansion using IL-7 and anti-γδTCR. • Analysis of IL-17A production post γδ T-cell expansion.
Expansion and Polarization of Human γδT17 Cells in vitro from ...
2024年1月1日 · These findings redefine human γδ T-cell subsets by delineating the Vδ2+ T-cell compartment into innate-like (Vγ9+) and adaptive (Vγ9-) subsets, which have distinct functions in microbial ...
Natural γδT17 cell development and functional ... - Cell Press
Natural IL-17-producing γδ T cells (γδT17 cells) are unconventional innate-like T cells that undergo functional programming in the fetal thymus. However, the intrinsic metabolic mechanisms of γδT17 cell development remain undefined.
Differential Roles of the mTOR-STAT3 Signaling in Dermal γδ T Cell ...
2019年6月4日 · Here, we show that the mechanistic or mammalian target of rapamycin (mTOR) and STAT3 pathways are activated in dermal γδT cells in response to innate stimuli such as interleukin-1β (IL-1β) and IL-23.
New approach to immunotherapy of clinical diseases discovered …
2022年5月25日 · T-cell can express T cell receptor, TCR to identify antigen and mediate immune response, and the T-cells can, by difference of their TCR peptide chain structure, be classified as αβT cell and...
results. (C) Cultured skin γδ T cell lines from C57BL/6 WT, IL-1RKO and MyD88KO mice were stimulated with IL-23 or IL-1β for 30 minutes. p-Stat3, p-AKT and p-S6 were examined by flow cytometry. Flow plots gated on CD3+ γδTCR+ cells are representative of at least two independent experiments with similar results. Plots from WT
Novel Insight Into the Molecular and Metabolic Mechanisms ... - PubMed
2019年12月3日 · Increasing evidence has demonstrated that IL-17-producing γδ T cells (γδ T17) play a tumor-promoting role in a series of cancers via various mechanisms in mice and human cancers, though the relationship between γδ T17 and human tumors has yet to be extensively characterized and established.