酵母人工染色体 - 百度百科
酵母人工染色体（Yeast artificial chromosomes，简称YAC），是一种能够克隆长达400Kb的DNA片段的载体，含有酵母细胞中必需的端粒、着丝点和复制起始序列，是细胞内具有遗传性质的物体，易被碱性染料染成深色，所以叫染色体（染色质）。
Yeast artificial chromosome - Wikipedia
Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces cerevisiae [1], which is then ligated into a bacterial plasmid. By inserting large fragments of DNA, from 100–1000 kb, the inserted sequences can be cloned and physically mapped using a process called chromosome walking.
酵母菌人工染色体 - 维基百科，自由的百科全书
酵母菌人工染色体 （Yeast artificial chromosome； YAC）是一种高容量 选殖载体，可嵌入的外来基因大小范围可为100kb至3000k。 此人工染色体系统含有 酵母菌 的 端粒 与 著丝粒，和复制起点。 可将特定的 DNA 置于端粒与著丝粒之间，使其在酵母菌体内复制。 进行选殖时可将目标序列以 限制酶 切下，再接合至酵母菌中，最早建立此系统的人是Murray & Szostak，于1983年建立。 YAC具有EcoRI和BamHI这两种限制酶可辨识的位置。
Yeast Artificial Chromosome (YAC) - National Human Genome …
1 天前 · Yeast artificial chromosome (YAC) is a human-engineered DNA molecule used to clone DNA sequences in yeast cells. YACs are often used in connection with the mapping and sequencing of genomes. Segments of an organism's DNA, up to one million base pairs in length, can be inserted into YACs.
酵母菌人工染色體 - 维基百科，自由的百科全书
酵母菌人工染色體（Yeast artificial chromosome；YAC）是一種高容量選殖載體，可嵌入的外來基因大小範圍可為100kb至3000k。 此人工染色體系統含有 酵母菌 的 端粒 與 著絲粒 ，和複製起點。
Yeast Artificial Chromosomes (YACs) - Microbe Notes
2022年4月2日 · Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast. It is a human-engineered DNA molecule used to clone DNA sequences in yeast cells. They are the products of a recombinant DNA cloning methodology to isolate and propagate very large segments of DNA in a yeast host.
(PDF) Yeast Artificial Chromosomes - ResearchGate
2012年1月1日 · A yeast artificial chromosome (YAC) containing a multigene cassette for expression of enzymes that enhance xylose utilization (xylose isomerase [XI] and xylulokinase [XKS]) was...
Yeast Artificial Chromosome - an overview - ScienceDirect
A Yeast Artificial Chromosome (YAC) is a vector specifically designed to carry large amounts of DNA, with the capacity to hold up to 2000 kb or 2 million base pairs. It mimics a natural chromosome in yeast cells and includes essential elements such as a yeast-specific origin of replication, a centromere recognition sequence, and telomere sequences.
Yeast Artificial Chromosome - an overview - ScienceDirect
Yeast artificial chromosomes (YACs) provide the largest insert capacity of any cloning system. This system, developed by Burke and Olson in 1987, supports the propagation of exogenous DNA segments hundreds of kilobases in length.
Construction and characterization of a yeast artificial chromosome ...
A yeast artificial chromosome (YAC) genomic library of Arabidopsis thaliana was constructed in a derivative of the vector pYAC4 which was modified to facilitate the production of end-specific probes for chromosome walking.