AAV 滴度衣壳蛋白试剂盒 - 金斯瑞 - GenScript
AAV2 Titer Capsid ELISA Kit （GenScript, L00942）针对AAV2病毒载体，提供AAV2标准品 (金斯瑞自产)以定量滴度。 金斯瑞L01035可检测包括但不限于：AAV1/2/5/6/8/9等血清型。 抗体原料/标准品可确保高纯度、高特异性、一致性和高稳定性。 线性范围宽/精密度高/准确度高。 检测时长约2.5小时。 金斯瑞AAV滴度衣壳蛋白ELISA试剂盒均是利用了两种抗AAV衣壳蛋白的单克隆抗体，它们可与衣壳蛋白的不同表位结合。 当标准品或样品加入捕获板时，AAV衣壳蛋白可以被 …
use purified AAV capsids, which most resemble the AAV vector of interest, as a standard. • When using CaptureSelect AAV biotinylated and HRP conjugated affinity ligands, the following AAV capsid concentration ranges can be used as a starting point for standard curve creation:
AAV ELISAs - PROGEN
As the industry gold standard you can rely on PROGEN’s AAV ELISAs to consistently deliver dependable capsid titer determination. You can select from a portfolio of AAV titration ELISAs and Xpress ELISAs for the most commonly used AAV serotypes.
Cell Biolabs QuickTiter™ AAV Titer ELISA Kit provides a quick and complete system to measure the titer of purified AAV viral samples; it provides sufficient reagents for up to 96 tests in a 96-well plate. Detection sensitivity is 6.25 x 108 viral particles (VP/mL) which is …
AAV2 Titer Capsid ELISA Kit - GenScript
GenScript AAV2 Titer Capsid ELISA Kit is designed for quantitatively measuring the total capsid titer of adeno-associated virus serotype 2 (AAV2) in testing samples.
Comprehensive characterization and quantification of adeno …
2021年2月4日 · Cp and Vg titers of AAV capsids are commonly measured independently using Capsid ELISA and qPCR, which can be time-intensive and highly variable, highlighting the need for more accurate and...
AAV ELISA How it works - PROGEN INC
How does the AAV ELISA work? The assay is based on the sandwich ELISA technique. A monoclonal antibody specific for a conformational epitope on assembled AAV capsids is coated onto a microtiter plate to capture fully assembled AAV particles from the specimen. Captured AAV particles are detected in a two step process.
Titration of AAV-2 particles via a novel capsid ELISA ... - Nature
1999年8月9日 · We demonstrate the rapid and reliable quantification of physical AAV-2 (adeno-associated virus type 2) particles via a novel ELISA based on a monoclonal antibody which selectively recognizes...
We show here that UV absorbance of denatured adeno-associated virus (AAV) vector provides a simple, rapid, and direct method for quantifying vector genomes and capsid proteins in solution. We determined the molar extinction coefficients of capsid protein to be 3.72 106 M1 cm1 at 260 nm and 6.61 106 M1 cm1 at 280 nm. For recombinant AAV vectors ...
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AAV ELISAs - PROGEN
As the industry gold standard you can always rely on PROGEN’s AAV ELISAs to consistently deliver reliable capsid titer determination. You can select from a portfolio of AAV quantification ELI-SAs and Xpress ELISAs for the most commonly used AAV serotypes (see page 2).
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