Cellular transduction mechanisms of adeno-associated viral vectors
Here, we review the cellular transduction mechanisms of rAAV vectors by breaking down the different steps leading to transgene expression. We also discuss strategies to overcome host restriction factors that act as barriers that limit the potential of …
Adeno-associated virus as a delivery vector for gene therapy
2024年4月3日 · Adeno-associated virus (AAV) has emerged as a pivotal delivery tool in clinical gene therapy owing to its minimal pathogenicity and ability to establish long-term gene expression in different...
Molecular Mechanism of AAV Transduction - Cell Press
Vectors based on the adeno-associated virus (AAV) are among the most promising tools for efficient and long term gene transfer into several tissues in vivo. Despite an increasing number of reports documenting the successful application of rAAV-mediated gene transfer, the molecular mechanisms governing rAAV genome processing after infection are ...
Structural and cellular biology of adeno-associated virus attachment ...
2020年1月1日 · In this chapter, we will discuss the interactions of AAV with its glycan and proteinaceous receptors and describe the host and viral components involved in AAV entry, which requires cellular attachment, endocytosis, trafficking to the trans -Golgi network and nuclear import. AAV serves as a paradigm for entry of nonenveloped viruses.
Recombinant Adeno-associated Virus Transduction and …
Instead of utilizing one mechanism in each phase of vector transduction, rAAV appears to employ multiple possible pathways toward transgene expression, in part dependent on rAAV serotype, dose, and target cell type.
Journey to the Center of the Cell: Tracing the Path of AAV Transduction
2020年10月15日 · Gene delivery vectors derived from adeno-associated virus (AAV) are the most widely used vectors for in vivo gene therapy. AAV possesses features that are attractive for clinical gene therapy and can be readily vectorized in a controlled fashion. In this review, we focus on the mechanisms of cellular transduction (see Glossary) of these vectors.
Intracellular trafficking of adeno-associated virus (AAV) …
2021年3月3日 · To transduce successfully a target cell, AAV has to overcome both systemic as well as cellular roadblocks. In this review, we discuss some of the most prominent cellular roadblocks that AAV...
Delivering AAV to the central nervous and sensory systems
Mechanism of AAV internalization and transduction. There are multiple steps to AAV transduction, which are laid out sequentially in Box 1 , Figure I . Transduction begins with capsid-cell adhesion (1), which is mediated by a large number of cell surface glycans, receptors and other moieties, including heparin sulfate proteoglycans (AAV2, 3b, 6 ...
Intracellular Trafficking of Adeno-Associated Virus (AAV) Vectors ...
To transduce successfully a target cell, AAV has to overcome both systemic as well as cellular roadblocks. In this review, we discuss some of the most prominent cellular roadblocks that AAV must get past to deliver successfully its therapeutic payload.
Adeno-associated virus (AAV) cell entry: structural insights - Cell …
2021年10月25日 · Adeno-associated virus (AAV) is the leading vector in emerging treatments of inherited diseases. Higher transduction efficiencies and cellular specificity are required for broader clinical application, motivating investigations of virus–host molecular interactions during cell entry.