SMARCAD1-mediated active replication fork stability …
We describe a hitherto unknown mechanism, by which the SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily-A containing DEAD/H box-1 (SMARCAD1) stabilizes active replication forks, that is essential to …
4D Visualization of replication foci in mammalian cells …
Replication forks active in parallel (GS/RFS/S-phase duration) 10,298±1: 8,216±0.0: Replicons active in parallel (active forks/2) 5,149: 4,108: Replicons per RFi (calculated replicons active in parallel/counted RFi) ... Image analysis: The brightness and colour of each image was adjusted with ImageJ 62. It should be considered that the ...
Nuclear actin polymerization rapidly mediates replication fork
2023年11月28日 · Nuclear actin polymerization mediates the engagement of fork remodelling factors and fork reversal. Active replication fork slowing upon DNA damage or mild replication interference was...
Single-molecule imaging reveals replication fork coupled ... - Nature
2021年5月5日 · We show that a small fraction of active replication forks spontaneously form G4s at newly unwound DNA immediately behind the MCM helicase and before nascent DNA synthesis. These G4s locally...
A DNA replication fork-centric view of the budding yeast DNA …
2022年11月1日 · Mec1 and Rad53 in budding yeast and ATR and Chk1 in metazoans are essential for cell viability and appear to have the most important roles during DNA replication. The DDR checkpoint regulates many cell processes including deoxyribonucleoside triphosphate (dNTP) production, transcription, and cell cycle progression [4], [8], [9], [10], [11].
Stabilization of expandable DNA repeats by the replication
2 天之前 · The integrity of the replication fork during elongation and coordination between leading and lagging strand synthesis are promoted by accessory replication fork proteins—including Ctf4 AND-1 and ...
MutSβ-MutLβ-FANCJ axis mediates the restart of DNA replication …
2024年2月7日 · R- loops impede DNA replication by inducing a fork remodeling process known as replication fork reversal , which involves replisome disassembly and the pairing of nascent DNA strands to form a DNA duplex protected by BRCA2-stabilized RAD51 filament .
Fork Cleavage-Religation Cycle and Active Transcription Mediate ...
2020年2月6日 · Here, we provide evidence that replication forks stalled by co-transcriptional R-loops can resume DNA synthesis via a multistep process involving cleavage of the stalled fork by SLX4-associated MUS81-EME1 endonuclease and subsequent fork religation catalyzed by RAD52 and the DNA ligase IV (LIG4)/XRCC4 complex.
Two-ended recombination at a Flp-nickase-broken replication fork
2 天之前 · Replication fork collision with a nicked DNA template was proposed to generate exclusively one-ended breaks. Elango et al. show that collision of a single replication fork with a Flp-induced nick can stimulate two-ended homologous recombination. Thus, the replisome can bypass some Flp-nicks, leaving a two-ended break in its wake.
The in vivo measurement of replication fork velocity and ... - Nature
2023年3月30日 · In this work, we quantitatively characterize known phenomena, detect brief, locus-specific pauses at ribosomal DNA loci in wild-type cells, and observe temporal fork velocity oscillations in...