Bacterial Artificial Chromosome (BAC) - National Human …
2 天之前 · A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells (for example, E. coli). BACs are often used in connection with DNA sequencing. Segments of an organism's DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs.
Application of a Bacterial Artificial Chromosome Modification …
Exactly controlled conditional gene expressing systems are crucial for genomic functional research, animal transgenesis and gene therapy. Bacterial artificial chromosomes (BACs) are optimal for harboring long fragments of genomic DNA or large cDNA up to 300 kb in size.
Bacterial Artificial Chromosome - an overview - ScienceDirect
Bacterial artificial chromosomes (BACs) are large genomic clones that are stably maintained in Escherichia coli. Because they are frequently large enough to contain an entire gene and its associated regulatory elements, BACs can serve as a source of …
Bacterial artificial chromosomes as analytical basis for gene ...
Bacterial Artificial Chromosomes (BACs) had been minimal components of various genome-sequencing projects, constituting perfect analytical basis for functional genomics. Here we describe an enhancer screening strategy in which BAC clones that cover ...
Construction and screening of BAC libraries made from ... - Nature
2007年6月28日 · Bacterial artificial chromosome (BAC) libraries are the large DNA insert libraries of choice and valuable tools for the map-based cloning of target quantitative trait loci, physical mapping,...
A Bacterial Artificial Chromosome Library for Sequencing the …
A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large average insert size (178 kb) has been constructed to provide the intermediate substrate for the international genome sequencing effort.
Bacterial Artificial Chromosome - an overview - ScienceDirect
The bacterial artificial chromosome (BAC) system is useful for creating a representation of the genomes of target species. The system is advantageous in that it can accommodate exogenous inserts that are very large (>100 kilobases, kb), thereby allowing entire eukaryotic genes (including flanking regulatory regions) to be encompassed in a ...
Bac to the future: The use of bac transgenic mice for neuroscience ...
2001年12月1日 · The development of methods for engineering bacterial artificial chromosomes (BACs), and for the efficient production of BAC transgenic mice, has allowed the design of new in vivo approaches...
Bacterial Artificial Chromosome - an overview - ScienceDirect
Bacterial artificial chromosomes (BACs) are plasmids used for cloning and stably maintaining large segments of foreign DNA in Escherichia coli. This is important in various types of analyses of mammalian and other genomes.
Generating Transgenic Mice from Bacterial Artificial Chromosomes ...
Bacterial artificial chromosome (BAC) transgenes direct gene expression at physiological levels with the same developmental timing and expression patterns as endogenous genes in transgenic animal models.
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