Immunohistochemistry for the detection of BRCA1 and BRCA2 ... - PubMed
Materials and methods: This systematic review aims to evaluate the current usage of IHC to detect BRCA1 and BRCA2 deficiencies in EOC. We searched and evaluated all primary literature on the use of IHC for evaluating BRCA1 and BRCA2 proteins expression in EOC.
BRCA immunohistochemistry for screening of BRCA mutation …
BRCA IHC is simple, inexpensive and a potential biomarker for BRCA dysfunction in EOC patients. The sensitivity of BRCA1 IHC for detection of germline BRCA1 mutation is less than 90% of BRCA1 patients (Garg et al., 2013, Meisel et al., 2014) and less than 70% in our study.
Immunohistochemistry for the detection of BRCA1 and BRCA2 …
The most consistent results on IHC for BRCA1 in ovarian cancer were obtained with the anti-BRCA1 mouse monoclonal clone MS110 antibody and a cut-off of 10% of positive cells. We recommend further studies on cut-off determination based on tumour sensitivity to drugs like PARP inhibitors.
Pathology Outlines - BRCA associated carcinoma
2024年4月16日 · Pathogenic variant of BRCA1 increases the risk of breast cancer by 65% (44 - 78%) and ovarian cancer by 39% (18 - 54%) by the age of 70; corresponding risks are 45% (31- 56%) and 11% (2.4 - 19%) for BRCA2 (Am J Hum Genet …
BRCA1 immunohistochemistry in a molecularly characterized …
Our goal was to assess whether immunohistochemical analysis (IHC) for BRCA1 is an effective method for the detection of BRCA1 dysfunction in molecularly characterized high-grade ovarian serous carcinoma.
The performance of BRCA1 immunohistochemistry for detecting …
2014年10月3日 · Given the potential importance of BRCA1 loss as a predictive and prognostic biomarker in high-grade serous ovarian cancer, we sought to evaluate the utility of BRCA1 immunohistochemistry (IHC) in screening for BRCA1 loss by …
BRCA locus-specific loss of heterozygosity in germline BRCA1 ... - Nature
2017年8月22日 · We evaluated whether the presence or absence of locus-specific LOH of BRCA1 was associated with retention of the protein as measured by BRCA1 IHC. All tumors demonstrating locus-specific LOH...
Brca1 haploinsufficiency promotes early tumor onset and …
2024年11月11日 · These findings reveal a previously unappreciated epigenetic effect of Brca1 haploinsufficiency in accelerating tumorigenesis, advancing our mechanistic understanding and informing potential ...
Immunohistochemical Expression of BRCA1 Protein, ER, PR and …
These findings raise the importance of identifying molecular profiling of ER, PR, Her2/neu and the state of BRCA1 expression as useful prognostic markers for BC patients before starting treatment and selecting proper persons for the genetic screening of the mutated BRCA1 oncoprotein.
BRCA1 immunohistochemistry in high-grade serous ovarian …
2012年5月20日 · We investigated the performance of BRCA1 immunohistochemistry (IHC) testing in HGS-OC with known BRCA1 mutation status. Methods: Eligible patients had HGS-OC, underwent surgery at a single institution between 1997 and 2010, and were tested for germline BRCA1 mutations under IRB-approved protocols.