研究发现,Ter-119、CD71、CD44等细胞表面分子标记可反映红细胞发育不同阶段,故本文以小鼠骨髓组织为例,使用3种带不同荧光标记对的抗体,结合细胞大小,对骨髓细胞中5个阶段的红细胞进行标记。 表1. 流式抗体. 8. 磷酸缓冲液 (10x PBS, Sangon biotech, catalog number: E607016-0500) 9. 磷酸缓冲液 (1x PBS) ( 见溶液配方) 10. 红细胞形成抗体混合液 ( 见溶液配方) 颈椎脱臼处死小鼠,从下腹部剪开小鼠腿部皮肤,小心剪开髋关节与踝关节,将小鼠腿部完整取出,分离肌肉,取出股骨与 …
Regulation of CD71+TER119+ erythroid progenitor cells by CD45
The CD71 + TER119 + progenitors are a major splenic population in neonates required for extramedullary erythropoiesis, to meet the high demand for red blood cells during growth. This population decreases as the mice mature, but this was not the case in CD45-deficient mice, which maintained a high level of these progenitors in the spleen into ...
Identification and Analysis of Mouse Erythroid Progenitors using the ...
Here we present a flow-cytometric method of identifying and studying differentiation-stage-specific erythroid progenitors and precursors, directly in the context of freshly isolated mouse tissue. The assay relies on the cell-surface markers CD71, Ter119, and on the flow-cytometric 'forward-scatter' parameter, which is a function of cell size.
The CD71/Ter119 erythroid subsets in mouse spleen. A
By combining Ter119 and CD71 expression, erythroid cells could be distinguished into four subpopulations: Ter119 med CD71 high , Ter119 high CD71 high Ter119 high CD71 med , and...
CD71 and TER119 can be used as markers of erythroid dif | Open-i
Early erythroid progenitors (the R1 population) express moderate levels of CD71 and are TER119−. Later progenitor cells (R2) express higher levels of CD71 but are still TER119−. As cells continue to divide and differentiate, TER119 expression is induced, and CD71 expression is down-regulated, as indicated by the R3, R4, and R5 populations.
Murine Bone Marrow Erythroid Cells Have Two Branches of ... - MDPI
2023年10月30日 · By evaluating CD71 and Ter-119 as sorting markers for murine erythroid cells and employing bulk NanoString transcriptomics, we discerned distinctive gene expression profiles between bone marrow and fetal liver-derived erythroid cells.
使用CD71/TER119流流式细胞仪检测小鼠红系祖细胞的识别和分析 …
2011年8月5日 · CD71/Ter119法可用于研究红系祖细胞在其体内红细胞生成应力的响应，例如，住在贫血小鼠或老鼠在低氧条件。 直接转基因的成年小鼠或胚胎组织，它也可以用来研究红系祖细胞，修改后的红细胞中的分子途径，以评估的具体作用。
使用CD71 / TER119流式细胞术鉴定和分析小鼠类红细胞祖细 …
2011年8月5日 · CD71 / Ter119测定法可用于研究类红细胞祖细胞在体内对红细胞生成应激的反应，例如在贫血小鼠或低氧条件下的小鼠中。 它也可用于直接在转基因成年小鼠或胚胎的组织中研究类红细胞祖细胞，以评估修饰的分子途径在红细胞生成中的特定作用。
Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells ...
Here, we report one population of tumor-inducible, erythroblast-like cells (Ter-cells) deriving from megakaryocyte-erythroid progenitor cells with a unique Ter-119 + CD45 − CD71 + phenotype. Ter-cells are enriched in the enlarged spleen of hosts bearing advanced tumors and facilitate tumor progression by secreting neurotrophic factor artemin ...
Flow cytometric analysis of CD71 and TER-119 expression
Like HCD-57 cells, J53Z1 cells are strongly positive for immature erythroid cell marker CD71 but negative for TER-119, and the level of CD71 is comparable to that seen in HCD-57 cells and...