A programmable dual-RNA-guided DNA endonuclease in …
2012年8月17日 · The dual-tracrRNA:crRNA, when engineered as a single RNA chimera, also directs sequence-specific Cas9 dsDNA cleavage. Our study reveals a family of endonucleases …
CRISPR–Cas9: A History of Its Discovery and Ethical …
The original biological function of CRISPR–Cas9 system is the protection of prokaryotes from mobile genetic elements, in particular viruses. Currently, CRISPR–Cas9 and related …
Genome engineering using the CRISPR-Cas9 system - Nature
2013年10月24日 · Here we describe a set of tools for Cas9-mediated genome editing via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, as …
CRISPR Timeline - Broad Institute
Moineau and colleagues demonstrated that CRISPR-Cas9 creates double-stranded breaks in target DNA at precise positions, 3 nucleotides upstream of the PAM (Garneau et al., 2010). …
CRISPR-Cas9: A History of Its Discovery and Ethical ... - PubMed
The original biological function of CRISPR-Cas9 system is the protection of prokaryotes from mobile genetic elements, in particular viruses. Currently, CRISPR-Cas9 and related …
The CRISPR tool kit for genome editing and beyond - Nature
2018年5月15日 · CRISPR-Cas9 is no longer just a gene-editing tool; the application areas of catalytically impaired inactive Cas9, including gene regulation, epigenetic editing, chromatin...
CRISPR/Cas9 therapeutics: progress and prospects
2023年1月16日 · Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating …
(PDF) CRISPR–Cas9: A History of Its Discovery and Ethical ...
2022年8月1日 · CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats CRISPR associated protein 9) is a groundbreaking genome editing tool that has revolutionized …
Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing
Guide RNA (gRNA) and CRISPR-associated (Cas-9) proteins are the two essential components in CRISPR/Cas-9 system. The mechanism of CRISPR/Cas-9 genome editing contains three …
The new frontier of genome engineering with CRISPR-Cas9
2014年11月28日 · We review the history of CRISPR (clustered regularly interspaced palindromic repeat) biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme …