The CellTiter-Glo® Assay is designed for use with multiwell plate formats, making it ideal for automated high-throughput screening (HTS) and cell proliferation and cytotoxicity assays. The homogeneous assay procedure (Figure 1) involves adding a single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium.
CellTiter-Glo® Luminescent Cell Viability Assay Protocol
The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method to determine the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells.
Promega’s new CellTiter-GloTM Luminescent Cell Viability Assay is a highly sensitive method for assaying cell proliferation and cytotoxicity. The assay uses the luciferase reaction to measure ATP, a global indicator of cellular metabolism.
CellTiter-Glo ® Luminescent Cell Viability Assay（CTG） - Promega
CellTiter-Glo®Luminescent Cell Viability Assay (CellTiter-Glo®发光法细胞活力检测试剂盒，简称CTG ) 是通过对 ATP 进行定量测定来检测培养物中活细胞数目的一种均质快速检测方法。 ATP 是活细胞新陈代谢的一个指标。 Promega的 CellTiter-Glo® 检测试剂盒为多孔板而设计，是进行自动化高通量筛选 (HTS)、细胞增殖和毒性分析的理想选择。 检测步骤就是将单一试剂 (CellTiter-Glo® 试剂 ) 直接加入含有血清的培养细胞中，无需洗涤细胞、去除培养基或进行多步加样操作。 在 …
(PDF) CellTiter-Glo™ luminescent cell viability assay
2001年1月1日 · We made serial two-fold dilutions of Jurkat cells in a 96 well plate in RPMI 1640 + 10% FBS according to the protocol in Technical Bulletin #TB288. Luminescence was recorded at 10 minutes using a...
The CellTiter-Glo® Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) and cell proliferation and cytotoxicity assays. The homogeneous assay procedure (Figure 1) involves adding a single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium.
Here we describe a method using the CellTiter-Glo® Assay adapted to assess viability of tumor cell lines embedded and treated in agarose in a 96-well format. Reagents and Cell Culture.
细胞活力检测方法大盘点 - MedChemExpress - 知乎 - 知乎专栏
CTG 发光法：基于高灵敏度生物发光检测技术，通过对三磷酸腺苷 (ATP) 进行定量以测定培养物中活细胞数目及细胞活力。 该实验方法可用三个字简单概括 “快、准、狠”，比 MTT 的处理速度快近 20 倍，节省时间近 4 小时。 CTG 特有的 均质检测法 即 “加样-混样-检测”，使用时，只需将本试剂等体积添加至培养细胞中即可进行检测。 不用感慨，细胞活力检测为什么不能如此简单？ 图 1. MTT、CCK8 与 CTG 法的实验流程图. 表 1. MTT、CCK8 与 CTG 法特点比较. 首先，来了 …
T Cell Proliferation CTG Assay | Charles River
Through our immune cell activation assays, we can assess the impact of candidate compounds on multiple super antigen-stimulated T cell receptor (TCR) engagement pathways. Proliferation endpoint measurement can be measured using the CellTiter-Glo ® (CTG) Assay readout.
Validation of in vitro assays in three-dimensional human dermal ...
2018年5月29日 · The bar graph shows the cell viability of 3D dermal construct analyzed by optimized CTG assay at different time points of 7 and 14 days of culture. Human in vitro dermal construct showed the filopodia-like morphology with dendritic extensions in the matrix and a uniform distribution of NHDF in different planes inside the matrix ( Figure 6(b) ).