细胞增殖，细胞毒性检测各种方法的原理和区别 - 知乎
MTT细胞增殖及细胞毒性检测试剂盒(MTT Cell Proliferation and Cytotoxicity Assay Kit)是一种非常经典的细胞增殖和细胞毒性检测试剂盒，被广泛应用于细胞增殖和细胞毒性的检测。
最主流的细胞活力检测方法 —— CTG(CELL TITER-GLO)发光法
2017年8月24日 · 检测细胞存活与增殖的方法主要包括观察DNA合成含量和检测细胞代谢活性两种，前者主要是DNA前体物质（胸腺嘧啶核苷类似物）掺入法，比如BRDU、EDU法；后者主要为MTT、XTT、MTS、CCK-8、WST-1及WST-8法等，在后者中现在最主流的就是CTG(CELL TITER-GLO)发光法，此法可 ...
CellTiter-Glo® Luminescent Cell Viability Assay Protocol
A protocol to determine the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells. This assay is designed for use in automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays.
cell proliferation and cytotoxicity. The assay uses the luciferase reaction to measure ATP, a global indicator of cellular metabolism. While providing a powerful tool for the life science researcher, the “add, mix, measure” format of the assay also satisfies the need for a simple and rapid assay for high-throughput screening. Introduction
T Cell Proliferation CTG Assay | Charles River - Charles River …
CTLA-4 blockade allows for activation and proliferation of more T cell clones and reduces Treg-mediated immunosuppression. PD-1 pathway blockade restores the activity of antitumor T cells that have become quiescent.
CTGF regulates cell proliferation, migration, and glucose metabolism ...
Connective tissue growth factor (CTGF), also known as CCN2, is a member of the CCN protein family of secreted proteins with roles in diverse biological processes. CTGF regulates biological functions such as cell proliferation, migration, adhesion, wound healing, and angiogenesis.
CTGF regulates cell proliferation, migration, and glucose metabolism ...
2021年3月10日 · CTGF regulates biological functions such as cell proliferation, migration, adhesion, wound healing, and angiogenesis.
Connective-tissue growth factor (CTGF) modulates cell
2002年7月22日 · The complete open reading frame of Xenopus CTGF was amplified by PCR from cDNA of stage 25 embryos using the following primers: forward 5′-AAA CTC GAG GAA CTA ATC TGC TGC TGT-3′ and reverse 5...
Macrophages are essential for CTGF-mediated adult β-cell proliferation …
2015年5月19日 · CTGF mediated β-cell mass proliferation involves the modification of several β-cell intrinsic characteristics. We now show that the immune system, specifically macrophages, is also required for effective CTGF-mediated β-cell mass expansion following injury.
Frontiers | Connective Tissue Growth Factor: From Molecular ...
2020年10月28日 · Connective tissue growth factor (CTGF) is a key signaling and regulatory molecule involved in different biological processes, such as cell proliferation, angiogenesis, and wound healing, as well as multiple pathologies, such as tumor development and tissue fibrosis.