Engineering the next-generation synthetic cell factory driven by ...
2024年7月1日 · This review focuses on summarizing the latest advancements of protein engineering in optimizing various aspects of synthetic cell factory, including: enhancing enzyme activity to eliminate production bottlenecks, altering enzyme selectivity to steer metabolic pathways towards desired products, modifying enzyme promiscuity to explore innovative ...
Advances in multi-enzyme co-localization strategies for the ...
2024年12月1日 · This review discusses recent developments and applications of enzyme co-localization strategies in constructing cell factories for efficient natural product production. It comprehensively describes enzyme-based compartmentalization, metabolic pathway-based compartmentalization, and synthetic organelle-based compartmentalization in improving ...
Systematic development of a highly efficient cell factory for 5 ...
2024年11月1日 · ALAS, a homodimeric pyridoxal-5′-phosphate (PLP)-dependent enzyme, serves as the key enzyme for the construction of a 5-ALA cell factory by condensing succinyl-CoA and glycine to form 5-ALA, CoA, and CO 2 [42]. The activity of this enzyme is crucial for determining the rate of 5-ALA production as it competes with succinyl-CoA synthetase to ...
Cell Factory Engineering: Cell Systems - Cell Press
Cell factory engineering is a broad and multifaceted discipline with applications in all areas of biotechnology. Here we present an overview of the area, and strategies generally applicable across cell types within the areas of production of native metabolites, heterologous expression of biosynthetic pathways, and heterologous expression of ...
Advances in multi-enzyme co-localization strategies for the ...
This article reviews recent progress and applications of these strategies in constructing cell factories for efficient natural product production. It comprehensively describes the applications of enzyme-based compartmentalization, metabolic pathway-based compartmentalization, and synthetic organelle-based compartmentalization in improving ...
Towards next-generation cell factories by rational genome-scale ...
2022年9月20日 · In addition to enzymes at branching points, targets include enzymes involved in biosynthesis or regeneration of cofactors that are important for cell factory performance, as well as...
Synthesizing ginsenoside Rh2 in Saccharomyces cerevisiae cell factory ...
2019年1月15日 · Based on this high PPD-producing chassis, we established a series of cell factories to produce ginsenoside Rh2, which we optimized by improving the C3–OH glycosylation efficiency. We increased...
Aspergillus oryzae as a Cell Factory: Research and Applications in ...
2024年3月26日 · Aspergillus oryzae, a biosafe strain widely utilized in bioproduction and fermentation technology, exhibits a robust hydrolytic enzyme secretion system. Therefore, it is frequently employed as a cell factory for industrial enzyme production.
Combined metabolic engineering and lipid droplets degradation …
2024年11月25日 · Background In microbial cell factories, substrate accessibility to enzyme is a key factor affecting the biosynthesis of natural products. As a robust chassis cells for biofuels and bioproducts, Saccharomyces cerevisiae also encounters the challenge since different enzymes and precursors are typically compartmentalized in different organelles. Such spatial separation could largely limit the ...
Developing Ganoderma lucidum as a next-generation cell factory …
2023年8月31日 · Ganoderma lucidum holds a huge reservoir of novel hydrolytic enzymes and therapeutic compounds, and can be transformed into a cell factory for proteins and nutraceuticals. Integrating heterologous genes into the homologous recombination (HR) pathway or utilizing nonhomologous end-joining inhibitors can improve the efficiency of HR in G. lucidum.