CellTiter-Glo ® Luminescent Cell Viability Assay（CTG） - Promega
CellTiter-Glo ® Luminescent Cell Viability Assay (CellTiter-Glo ® 发光法细胞活力检测试剂盒，简称CTG ) 是通过对 ATP 进行定量测定来检测培养物中活细胞数目的一种均质快速检测方法。ATP 是活细胞新陈代谢的一个指标。
CellTiter-Glo® Luminescent Cell Viability Assay Protocol
The CellTiter-Glo® Assay is designed for use with multiwell plate formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding a single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium.
The CellTiter-Glo® Assay is designed for use with multiwell plate formats, making it ideal for automated high-throughput screening (HTS) and cell proliferation and cytotoxicity assays. The homogeneous assay procedure (Figure 1) involves adding a single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium.
最主流的细胞活力检测方法 —— CTG(CELL TITER-GLO)发光法
2017年8月24日 · 检测细胞存活与增殖的方法主要包括观察DNA合成含量和检测细胞代谢活性两种，前者主要是DNA前体物质（胸腺嘧啶核苷类似物）掺入法，比如BRDU、EDU法；后者主要为MTT、XTT、MTS、CCK-8、WST-1及WST-8法等，在后者中现在最主流的就是CTG(CELL TITER-GLO)发光法，此法可 ...
技术 | 国内外主流 AI 统一推荐的最经典的3D细胞活力检测方法！_ …
2024年3月20日 · Promega的CellTiter-Glo ® Luminescent Cell Viability Assay（简称CTG）是基于ATP的细胞活力检测，在国内外科研及制药企业中被广泛应用，是公认的细胞活力检测“金标准”。 3D版的CellTiter-Glo ® 进行了优化，试剂裂解能力更强，可以更高效的渗透至打的3D培养物或细胞球内。 下图. CellTox 绿色荧光染料可以结合于膜完整性受损的细胞的双链 DNA， 使用CellTiter-Glo ® 3D和A 检测试剂处理细胞后，应用激光共聚焦显微镜拍照。 绿色荧光染色显示裂解的细 …
The CellTiter-Glo® Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) and cell proliferation and cytotoxicity assays. The homogeneous assay procedure (Figure 1) involves adding a single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium.
T Cell Proliferation CTG Assay | Charles River
Through our immune cell activation assays, we can assess the impact of candidate compounds on multiple super antigen-stimulated T cell receptor (TCR) engagement pathways. Proliferation endpoint measurement can be measured using the CellTiter-Glo ® (CTG) Assay readout.
(PDF) CellTiter-Glo™ luminescent cell viability assay
2001年1月1日 · Cell viability assays are critical in evaluating the health of cells in response to drug treatment or other stimuli to better understand how these factors effect...
Promega’s new CellTiter-GloTM Luminescent Cell Viability Assay is a highly sensitive method for assaying cell proliferation and cytotoxicity. The assay uses the luciferase reaction to measure ATP, a global indicator of cellular metabolism.
This document describes automation of the CellTiter-Glo® Luminescent Cell Viability Assay(a,b,c) on the Beckman Coulter Biomek ® 2000 and Biomek FX and Hamilton MICROLAB ® STAR automated liquid-handling workstations.
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