DNA损伤检测技术，你知道多少？ - 知乎专栏
2020年1月7日 · DNA损伤过程中可形成某些特定的损伤产物，可通过检测损伤产物（磷酸化H2AX、8-OHdG等）来评估DNA损伤。 也可标记损伤DNA，如末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸标记（terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling，TUNEL），通过标记物的检测间接反映DNA的损伤程度。 1.双链断裂标志物磷酸 …
Strategies for the evaluation of DNA damage and repair mechanisms …
As the assay is able to detect the DNA fragments with fluorescence or radioactivity, microscopy techniques, FCM, photo-multipliers and charge coupled device arrays may be used to detect and quantify DNA damage caused by apoptosis .
DNA损伤检测试剂盒 (γ-H2AX免疫荧光法) - Beyotime
碧云天生产的DNA损伤检测试剂盒(γ-H2AX免疫荧光法)，英文名为DNA Damage Assay Kit by γ-H2AX Immunofluorescence，是通过免疫荧光染色检测DNA损伤标记物γ-H2AX (即磷酸化的H2AX)的含量以确认DNA是否损伤的检测试剂盒。
Methods and applications of genome-wide profiling of DNA damage …
2024年6月25日 · Over the past few years, a comprehensive toolbox of genome-wide methods has been developed for the detection of DNA damage and rare mutations at single-nucleotide resolution in mammalian cells....
The comet assay: a method to measure DNA damage in individual …
2006年6月27日 · We present a procedure for the comet assay, a gel electrophoresis–based method that can be used to measure DNA damage in individual eukaryotic cells. It is versatile, relatively simple to...
An optimized comet-based in vitro DNA repair assay to assess ... - Nature
2020年11月16日 · In our comet-based in vitro repair assay, a protein lysate is extracted from cells or tissues and incubated with damage-containing substrate DNA consisting of gel-embedded nucleoids from...
Exploring DNA Damage and Repair Mechanisms: A Review with …
2024年1月24日 · The review addresses key questions, including the major types of DNA damage, common DNA repair mechanisms, the availability of reliable databases for DNA damage and associated diseases, and the predominant computational research methods for enzymes involved in DNA damage and repair.
Comet assay to measure DNA repair: approach and applications
2014年8月24日 · In a biochemical approach, substrate nucleoids containing specific DNA lesions are incubated with cell extract; repair enzymes in the extract induce breaks at damage sites; and the breaks are measured with the comet assay. The nature of the substrate lesions defines the repair pathway to be studied.
DNA damage by oxidative stress: Measurement strategies for …
2018年2月1日 · Some of the most widely used tools to measure oxidative DNA damage are the comet assay, PCR-based assays, immunoassays, and mass spectrometry-based approaches.
DNA damage measurements within tissue samples with Repair …
We verified the ability of the RADD assay to detect DNA damage in tissue by exogenously inducing DNA damage with X-rays and restriction enzymes. We also showed that RADD can be multiplexed with antibodies to detect cell cycle markers or other proteins of interest.
- 某些结果已被删除