Pairs with endothelial cell density (ECD) below 2000 cells/mm2 and more than 10% difference between corneas at day (D)2 were excluded.

Download scientific diagram | Representative image of a cell and its respective masks. a) Brightfield and nuclear image (purple color) of an endothelial cell acquired by Amnis ImageStream...

We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like...

2015年12月23日 · Figure 2. Brightfield images of representative BOEC cultures. (A) Outgrowth colonies appear in cultures between days 7 and 14. Colonies present as collections of endothelial-like cells, which are arranged in a cobblestone monolayer and proliferate radially out from a …

Generation and characterization of vaschamcardioids (vcCOs) with endothelial cells from human induced pluripotent stem cell (hiPSCs). (A) Schematic diagram of the 3D culture method for generation of chambered cardiac organoids with vascular system.

2015年6月22日 · Our studies revealed the presence of tunnelling nanotubes in microvascular endothelial cells (HMEC-1). The TNTs were studied with live cell imaging, environmental scanning electron microscopy...

2021年6月5日 · Endothelial cells (ECs) have emerged as key pathogenic players in cardiac disease due to their proximity with cardiomyocytes. Induced pluripotent stem cells (iPSCs) have been employed to generate ECs. However, it may be more clinically relevant to transdifferentiate fibroblasts into ECs directly wit …

2021年12月14日 · Purpose: Corneal endothelial cells (CECs) serve as a barrier and foothold for the corneal stroma to maintain the function and transparency of the cornea. Loss of CECs during aging or disease states leads to blindness, and cell replacement therapy using either donated or artificially differentiated CECs remains the only curative approach.

2015年6月22日 · TNT-like structures connect endothelial cells (HMEC-1). (a) Brightfield images of TNTs in HMEC-1. TNTs connect cells at distances over 100 μm. White lines are placed parallel to TNT-like structures and illustrate the length of the corresponding TNTs. Scale bars, 20 μm.

2016年9月19日 · Reconstructed images from a T-cell attached to the endothelial cell (green circle) and a flowing T-cell (red circle) are captured.