In the past, the majority of the quantitative test for glucose determination depended upon the oxidation of glucose by hot, alkaline copper solutions or solutions of potassium ferricyanide. These were replaced by the ortho-toluidine test and later by enzyme methods. Enzymatic methods give “true” glucose determination because of the high

2023年6月1日 · Herein, a colorimetric assay for detection of glucose by enzymatic etching of triangular gold nanosheets is presented. It was demonstrated by a combination of experimental and characterization means that both enzymatic reaction and H 2 O 2 etching affect the LSPR peaks of gold nanoparticles, which in turn affects the sensitivity of the sensor.

2021年7月21日 · In this study, Amplex Red (AR), as both indicator and mediator, was investigated to detect glucose in presence of glucose oxidase (GOx) enzymes using colorimetric and electrochemical methods. Without using any advanced techniques and sophisticated nanomaterials, 1 μM glucose can be easily detected through simply detecting the solution color ...

2015年12月1日 · In this review we provide an overview of enzymatic glucose detection systems, and discuss their relative strengths and weaknesses. We also examine the assorted chromatographic systems and electrophoretic-based systems (HPLC, GC, CZE) and associated detectors which may be used for glucose detection and analysis.

Blood sugar or blood glucose is a basic test used to assess glucose metabolism disorders such as diabetes mellitus or hypoglycaemia. Testing is done by two methods which are chemistry and enzyme. However, chemical methods are currently unavailable for measuring non-specific and high time-consuming

2018年2月17日 · In this work, a multilayer-modified paper-based colorimetric sensing platform with improved color uniformity and intensity was developed for the sensitive and selective determination of uric acid and glucose with smartphone as signal readout.

2019年2月5日 · A colorimetric method for glucose determination was developed based on etching of gold nanorods (GNRs) using CQDs as peroxidase mimetic. The glucose oxidase-catalyzed oxidation of glucose leads to the generation of H2O2 which oxidizes added iodide under formation of elemental iodine under the catalytic action of CQDs.

Glucose LiquiColor® Test Procedure No. BD184 For the Quantitative Enzymatic- Colorimetric Determination of Glucose in Serum, Plasma or CSF Summary and Principle chemical procedures employed in clinical laboratory medicine.1 Glucose oxidase methodology was introduced by Keilin and Hartree2 in 1948. Keston3 later reported use of the combined glucose

This document describes a GOD-PAP enzymatic colorimetric method for determining glucose concentration. Glucose is oxidized by glucose oxidase to form hydrogen peroxide, which then reacts with phenol and 4-aminophenazone under catalysis of peroxidase to form a red-violet quinoneimine dye.

The Glucose Detection research-use-only kit is a colorimetric assay designed for the quantification and detection of glucose in serum, plasma, urine, buffers and tissue culture media. This complete, ready-to-use kit includes clear 96-well plate (s), glucose standard, assay buffer, and other components to perform the assay.