Immunogold labeling or immunogold staining (IGS) is a staining technique used in electron microscopy. [2] This staining technique is an equivalent of the indirect immunofluorescence technique for visible light.

免疫胶体金技术 (Immune colloidal gold technique) 是以胶体金作为示踪标志物应用于抗原抗体的一种新型的免疫标记技术，英文缩写为：GICT。 胶体金是由 氯金酸 （HAuCl4）在 还原剂 如 白磷 、抗坏血酸、枸橼酸钠、鞣酸等作用下，聚合成为特定大小的金颗粒，并由于静电作用成为一种稳定的胶体状态，称为胶体金。 免疫胶体金技术 (Immune colloidal gold technique) 是以胶体金作为示踪标志物应用于抗原抗体的一种新型的免疫标记技术，英文缩写为：GICT。 胶体金是由 …

免疫 金标记技术 (Immunogold labelling techique) 主要利用了金颗粒具有高电子密度的特性，在金标蛋白结合处，在显微镜下可见黑褐色颗粒，当这些标记物在相应的配体处大量聚集时，肉眼可见红色或粉红色斑点，因而用于定性或半定量的快速 免疫检测 方法中，这一 ...

免疫金标记技术(Immunogold labelling techique) 主要利用了金颗粒具有高电子密度的特性，在金标蛋白结合处，在显微镜下可见黑褐色颗粒，当这些标记物在相应的配体处大量聚集时，肉眼可见红色或粉红色斑点，因而用于定性或半定量的快速免疫检测方法中，这一 ...

2022年2月10日 · 免疫金银染色（immunogold-silver staining，IGSS）是将 IGS 和银 显影法相结合，结合于抗原部位的金颗粒还原银离子生成的银原子围绕金颗粒形成「银壳」，「银壳」本身具有催化作用，催化更多的银离子还原沉积并促使「银壳」不断增大，从而能加强细胞或组织抗原 ...

Immuno-gold staining exploits antigen-antibody reactions and is used to investigate locations and interactions of components in the ultrastructure of tissues, cells, and particles. These methods are increasingly used with advanced technologies, such as correlative light and electron microscopy and cryo-techniques.

Immunogold electron microscopy is a classic high-resolution method for the selective localization of macromolecules in the context of cells and subcellular structures. Specific antibodies are used to affix small particles of colloidal gold, which are easily visible in the electron microscope, to the macromolecule of interest.

Presented here is a generic method for immunogold labelling for scanning electron microscopy, using two examples of specimens: isolated nuclear envelopes and the cytoskeleton of mammalian culture cells.

2013年6月25日 · Nature Neuroscience - Immunogold techniques allow target proteins to be localized subcellularly quantitatively and with high precision. In this review, the authors discuss applications of these...

Immunogold Labeling Method for Electron Microscopy (for Ultrathin Sections) (All incubations are at room temperature other than those indicated.) Fixation, embedding, and sectioning: