Indole Test- Principle, Reagents, Procedure, Result ...
2022年8月10日 · Principle of Indole Test. Tryptophan is an amino acid that can undergo deamination and hydrolysis by bacteria that express tryptophanase enzyme. Indole is generated by reductive deamination from tryptophan via the intermediate molecule indolepyruvic acid.
Indole test - Wikipedia
The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to convert tryptophan into indole. This division is performed by a chain of a number of different intracellular enzymes , a system generally referred to as " tryptophanase ."
To test for the presence of indole, a by-product of tryptophan metabolism, 5 drops of Kovács reagent should be added to the top of the deep. A positive indole test is indicated by the formation of a red color in the reagent layer on top of the agar deep within seconds of adding the reagent.
Indole Test- Principle, Media, Procedure, Types, Results, Uses
2022年1月23日 · Indole test is a biochemical test conducted on bacterial species to detect their ability to produce indole from tryptophan in the presence of a group of enzymes called ‘tryptophanase’. It is a qualitative test that tests the conversion of tryptophan into indole.
Indole Test: Principle, Procedure, Results - Microbe Online
Indole test is used to determine the ability of an organism to split amino acid tryptophan to form the compound indole. It is used as part of the IMViC procedures to differentiate members of the family Enterobacteriaceae. Tryptophan is hydrolyzed by tryptophanase to produce three possible end products; indole, pyruvate, and ammonia.
Indole Test - ASM.org
2019年8月12日 · The indole test screens for the ability of an organism to degrade the amino acid tryptophan and produce indole. It is used as part of the IMViC procedures, a battery of tests designed to distinguish among members of the family Enterobacteriaceae.
What is Indole Test? Definition, Principle, Reagents, Methods ...
Indole test is an IMViC test procedure that aims to distinguish different members of bacteria (indole positive or indole negative organisms) based on the splitting of tryptophan present in the medium into an end product indole. It uses Kovacs reagent to classify the members of Enterobacteriaceae.