Construction of shRNA lentiviral vector - PMC - PubMed Central …
The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, (3) using packaging cells to produce shRNA lentivirus, …
慢病毒 shRNA 和 miRNA 分析 | Thermo Fisher Scientific - CN
将对 shRNA 或 miR RNAi 编码的双链 DNA 寡核苷酸克隆成 BLOCK-iT 入门 (shRNA) 或表达 (miR RNAi) 载体之一。 通过 Gateway 重组将 RNAi 盒转染成腺病毒（仅 shRNA）或慢病毒目的载体。
Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of …
We provide suggestions for designing shRNA targets and controls, a protocol for sequencing through the secondary structure of the shRNA hairpin structure, and protocols for packaging and delivery of shRNA lentiviral particles. Using real-time PCR and functional assays we demonstrate the successful knockdown of ASC, an inflammatory adaptor molecule.
Short hairpin RNA - Wikipedia
Lentiviral delivery of shRNA and the mechanism of RNA interference in mammalian cells. A short hairpin RNA or small hairpin RNA (shRNA/Hairpin Vector) is an artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi).
A streamlined method for the design and cloning of shRNAs into …
Short hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to infect most mammalian cell types with high efficiency, regardless of proliferation state.
A new design of a lentiviral shRNA vector with inducible co …
2015年12月8日 · In this study, we evaluated key elements required for AGO2-mediated enhancement of gene silencing and developed a novel lentiviral shRNA vector with inducible co-expression of AGO2.
Plasmid-based shRNA lentiviral particle production for RNAi ...
Here, we present our complete optimized workflow for lentiviral particle production starting from bacterial glycerol stocks through purification of the pLKO.1 expression plasmid containing a simple stem loop shRNA cassette for each clone and finally transfection of the three plasmid-based system into HEK293T cells to demonstrate its enhancement ...
Construction of shRNA lentiviral vector - PubMed
This article summarized shRNA lentiviral vector methods generally used in research laboratories. The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, (3) using packaging cells to produce shRNA lentivirus, and ...
TRC Lentiviral shRNA - Horizon Discovery
The pLKO1 lentiviral vector enables efficient transduction of primary and non-dividing cells, such as neuronal cells making it easy to perform RNAi studies in these hard to transfect cell lines. Stable selection is also possible using the puromycin selectable marker.
Genome-scale loss-of-function screening with a lentiviral RNAi
2006年8月23日 · Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and...
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