Live-cell imaging - Wikipedia
Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging was pioneered in the first decade of the 21st century.
Live-cell imaging powered by computation - Nature
2024年2月20日 · We specifically discuss how computational approaches can improve the signal-to-noise ratio, spatial resolution, temporal resolution and multi-colour capacity of live-cell imaging.
LIVECell—A large-scale dataset for label-free live cell ... - Nature
2021年8月30日 · Here, we present LIVECell, a large, high-quality, manually annotated and expert-validated dataset of phase-contrast images, consisting of over 1.6 million cells from a diverse set of cell...
Label-free live cell recognition and tracking for biological ... - Nature
2024年10月7日 · Label-free, live cell recognition (i.e. instance segmentation) and tracking using computer vision-aided recognition can be a powerful tool that rapidly generates multi-modal readouts of cell...
Live-cell imaging - PMC - National Center for Biotechnology …
2014年10月31日 · There are many factors that need to be considered when performing live-cell imaging in order to maintain cellular health such as: imaging modality, media, temperature, humidity, PH, osmolality, and photon dose.
Overview of Live-Cell Imaging: Requirements and Methods Used
2012年8月21日 · Live-cell imaging using fluorescent proteins and fluorescent probes is a powerful tool for cell biology, but a need persists for noninvasive techniques to study tissue and cell dynamics without introducing chemical or genetically encoded probes.
Introduction to Live-Cell Imaging - Leica Microsystems
2012年4月3日 · Live-cell imaging provides spatial and temporal information of dynamic molecular events in single cells, cellular networks (in situ) or even whole organisms (in vivo). These features make live-cell imaging a requisite technique for addressing physiological questions in cell biology, cancer research, developmental biology and neuroscience.
Live cell imaging and systems biology - PMC - National Center …
In the present review, we provide an introduction into the capabilities of live cell imaging relevant for systems biology, and also detail concrete examples of how imaging methods might be profitably applied to analyze a specific molecular network, namely NF-κB.
Live Cell Imaging and in vivo Cell Tracking in Tissues
2023年11月16日 · Optical microscopy for live cell imaging constitutes a scientific methodology that leverages light encompassing wavelengths within the visible spectrum. Employing specialized methodologies, it affords the real-time examination and analysis of …
Live-cell Imaging by Super-resolution Confocal Live Imaging …
Many questions in cell biology can be solved by state-of-the-art technology of live cell imaging. One good example is the mechanism of membrane traffic, in which small membrane carriers are rapidly moving around in the cytoplasm to deliver cargo proteins between organelles.
Bridging live-cell imaging and next-generation cancer treatment
2023年9月13日 · By providing spatial, molecular and morphological data over time, live-cell imaging can provide a deeper understanding of the cellular and signalling events that determine cancer response to...
Live-cell imaging: Deeper, faster, wider | Science | AAAS
2018年3月27日 · The latest in live-cell microscopy—multiphoton imaging, light-sheet techniques, and technology borrowed from Raman spectroscopy—allow researchers to study living cells in more detail with less effort.
Live Cell Imaging: Methods and Protocols | SpringerLink
In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology.
Live Cell Imaging - CSH Protocols
New analytical options include live high-throughput/high-content screening in mammalian cells and computational analysis of live cell data. The manual presents hands-on techniques as well as background material, and can serve as a text in advanced courses.
Live Cell Imaging: Methods and Protocols | SpringerLink
Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research ...
Super-resolution 3D live cell imaging | Nature Methods
2021年3月5日 · 3D pRESOLFT enables volumetric live cell imaging with high spatial and temporal resolution. Super-resolution imaging of fixed cells in 3D is well established.
Imaging cell biology - Nature
2022年7月27日 · Imaging technologies drive discovery in cell biology. Innovations in microscopy hardware, imaging methods and computational analysis of large-scale, complex datasets can increase imaging...
Live-Cell Imaging: A Balancing Act Between Speed, Sensitivity, …
2022年11月30日 · As research models, spheroids and organoids offer a compromise between 2D cell lines and living animals. Live-cell imaging has been essential to probe their complex morphology and demonstrate that they faithfully reflect their in vivo counterparts.
Light Microscopy Techniques for Live Cell Imaging | Science - AAAS
2003年4月4日 · When selecting which system to use for imaging living cells, one should consider three things: sensitivity of detection, speed of acquisition, and the viability of the specimen.
Super-resolution microscopy demystified - Nature Cell Biology
2019年1月2日 · A key task is to improve SRM’s live-cell imaging capabilities by increasing temporal resolution and lowering photon burden.