At the Core of Microglial Signaling: γ-Secretase - ALZFORUM
2023年11月23日 · To find out, Hou and Zielonka analyzed single-cell transcriptomes of 8,600 H9 cultured microglial cells before and after semagacestat treatment. They saw two cell clusters: homeostatic and DAM-like. Among the untreated, 36 percent of the cells were homeostatic, 64 percent were DAMs, the latter possibly a reflection of microglia being in culture.
A Match Made in Microglia? ApoE and Aβ Click in Lysosomes, …
2024年10月30日 · This Aβ preparation most likely included a mixture of soluble and aggregated Aβ species. Microglial cells internalized the Aβ, promoting aggregation in the presence of ApoE fibrils, and this effect was consistent across BV2, primary, and iPSC-derived microglia, reinforcing the reliability of the findings beyond iPSC models.
In Amyloid and Tangle Models, APOE4 Paralyzes Microglia
2023年10月26日 · Liu and Wang et al. use a microglial-specific knock-in of the human APOE3 or APOE4 alleles in the context of an APOE-/- background, whereas Yin and Rosenzweig et al. specifically deleted only microglial APOE in the context of the humanized knock-in APOE mouse model. Both papers demonstrate conclusively that microglial APOE does contribute to ...
I’m Open! Exposed Enhancers Reveal Masters of Microglial Moods
2024年8月16日 · For example, in response to a TREM2-activating antibody, IRF3 and E2F emerged as potential master regulators of microglial transitions into interferon-producing and proliferative states, respectively. Yet, after a brief spell of transcriptional upheaval, the cells settled back into their calm, “undosed” state within days.
Some Plasma Proteins Bolster Phagocytosis and Metabolism in …
2024年10月29日 · ApoA-I also revved microglial phagocytosis, including of fluorescently labeled, fibrillar Aβ, suggesting these microglia might help rein in amyloid plaques. Finally, in cells isolated from ApoA-I knockout mice, phagocytosis of fluorescently labeled myelin flagged when microglia came from the hypothalamus, but not the cortex.
Easing Microglial Brakes Alleviates Amyloid Pathology in Mice
2024年4月11日 · “This is a very interesting paper on microglial mechanisms in AD pathogenesis with implications for potential drug development,” Yadong Huang, University of California, San Francisco, told Alzforum. Leukocyte Ig-like receptors (LILRs) are a group of 11 microglial markers; five activate the cells, five inhibit them.
T Cells in the Brain Goad Microglia to Muddle Myelin
2024年6月29日 · CD8+ T cells induce myelin pathology by microglial activation; 4. depletion of T cells or microglia, and blocking interferon or JAK signaling, protects against myelin pathology. We and other groups showed that the removal and modulation of T cells, or T cell-mediated microglia function, rescued many aspects of neurodegeneration, particularly ...
Polygenic Scores Point to Microglia, Astrocytes in Alzheimer's ...
2023年12月15日 · Tally of microglial variants tied to neuritic plaques, tau tangles, and cognitive decline. Researchers led by Hyun-Sik Yang of Brigham and Women’s Hospital in Boston deployed a cell-type-specific version of polygenic risk scores, by tallying the burden of AD risk variants near genes active in specific cell types.
Long Live the Microglia! Studies Trace Their Lifespans in Mice and ...
2017年8月30日 · Diego Gomez-Nicola of the University of Southampton in the U.K., who had previously reported that microglia turn over rapidly, echoed Butovsky’s concerns about selective labeling of microglial cells, wondering if some microglial subtypes might be more prone to the tamoxifen-induced recombination used to turn on tdTomato (Askew et al., 2017 ...
Circulatory proteins shape microglia state and boost phagocytosis ...
2024年10月29日 · Proteomic analysis of the plasma-positive microglia identified over 200 proteins enriched in these cells, with an overlap between the RNA and protein levels of numerous targets. At a functional level, the plasma-positive microglia were more metabolically active, with increased phagocytic activity, demonstrating how functionally distinct this ...