A single-cell molecular map of mouse gastrulation and early ...
2019年2月20日 · The 48 h of mouse embryonic development from embryonic day (E) 6.5 to E8.5 encompass the key phases of gastrulation and early organogenesis, when pluripotent epiblast cells diversify into...
Mouse gastrulation: Coordination of tissue patterning, …
2020年9月1日 · During mouse embryonic development a mass of pluripotent epiblast tissue is transformed during gastrulation to generate the three definitive germ layers: endoderm, mesoderm, and ectoderm. During gastrulation, a spatiotemporally controlled sequence of events results in the generation of organ progenitors and positions them in a stereotypical ...
Mouse gastrulation: the formation of a mammalian body plan
1997年11月1日 · In the mouse, gastrulation is a constantly changing, three-dimensional puzzle that has unique features when compared to non-mammalian and even other mammalian embryos. After implantation in the uterus, the mouse embryo initially develops as a cylindrical structure that is quite different from most mammalian embryos.
Gene function in mouse embryogenesis: get set for gastrulation
2007年3月27日 · During early mouse embryogenesis, temporal and spatial regulation of gene expression and cell signalling influences lineage specification, embryonic polarity, the patterning of tissue...
A single-cell time-lapse of mouse prenatal development from
2024年2月14日 · To progress towards a more comprehensive, continuous view of transcriptional dynamics throughout prenatal development, we sought to deeply sample single nuclei from mouse embryos precisely...
A single-embryo, single-cell time-resolved model for mouse gastrulation ...
2021年5月27日 · Here, we introduce a temporal model for mouse gastrulation, consisting of data from 153 individually sampled embryos spanning 36 h of molecular diversification. Using algorithms and precise timing, we infer differentiation flows and lineage specification dynamics over the embryonic transcriptional manifold.
Mouse embryo model derived exclusively from embryonic stem …
We utilized a recently established time-resolved model of mouse gastrulation consisting of ∼68,000 single cells derived from 287 individually processed embryos spanning egg cylinder stage to early somitogenesis (Figure 3A, Mittnenzweig et al., 2021), which (1) enables a quantitative evaluation of transcriptional states; and (2) describes the ...
Time-aligned hourglass gastrulation models in rabbit and mouse
2023年6月8日 · Here, we compare rabbit and mouse time-resolved differentiation trajectories to revisit this model at single-cell resolution. We modeled gastrulation dynamics using hundreds of embryos sampled between gestation days 6.0 and 8.5 and compared the species using a framework for time-resolved single-cell differentiation-flows analysis.
Multi-omics profiling of mouse gastrulation at single-cell ... - Nature
2019年12月11日 · Single-cell mapping of chromatin accessibility, DNA methylation and RNA expression during gastrulation in mouse embryos shows characteristic epigenetic changes that accompany formation of the...
Signaling regulation during gastrulation: Insights from mouse embryos ...
2020年1月1日 · WNT, BMP, Nodal and FGF signals cooperate to initiate gastrulation and germ layer specification. Disruption of any of these pathways results in cessation of gastrulation and early embryonic lethality. Here we discuss the current state of knowledge of the roles and regulation of these signals during gastrulation, gleaned from mouse embryos and PSCs.