Sequestration of PRMT1 and Nd1-L mRNA into ALS-linked FUS
2017年1月17日 · To test whether Nd1-L mRNA is sequestered into FUS-R521C complexes, we performed mRNA-protein pull-down assay with biotinylated Nd1-L mRNA and streptavidin-agarose beads using HEK293T...
Active RNA interference in mitochondria | Cell Research - Nature
2020年8月17日 · We thus tested multiple shRNAs against mtDNA-encoded ND1, COXI, COXII, and COXIII in transfected HEK293T cells and detected recordable, but somewhat variable effects (likely due to different ...
Nonstop mRNAs generate a ground state of mitochondrial gene
2022年11月18日 · Together, our collective data reveal sources by which nonstop mRNAs arise in mitochondrial gene expression and can act as a negative regulator of protein synthesis. In turn, this underpins a selective pressure to maintain the framework of a bacterial ribosome rescue pathway that is mediated by MTRFR.
NAD + precursor supplementation prevents mtRNA/RIG-I …
2023年3月13日 · We find that cisplatin exposure causes cytosolic leakage of mitochondrial RNA (mtRNA) and activation of the cytosolic pattern recognition receptor retinoic acid-inducible gene I (RIG-I), both of...
Mitochondrial complex I subunit MT-ND1 mutations affect disease ...
2024年4月15日 · Upregulation of normal MT-ND1 expression or ectopic expression of MT-ND1 has been shown to salvage respiratory chain CI defects caused by MT-ND1 mutations and can be applied in the treatment of related diseases. A CRISPR-free RNA-free DDDA-derived cytosine base editor (DdCBE), reported in 2020, is able to precisely manipulate mtDNA to make ...
Clarifying the ability of NeuroD1 to convert mouse microglia into ...
2021年12月15日 · In our previous article (Matsuda et al., 2019), we demonstrated that NeuroD1 (ND1) induces microglia-to-neuron (MtN) conversion both in vitro and in vivo. For in vitro experiments, we used primary cultured mouse microglia, almost all of which are Iba1 +, CD68 +, and CD11b + but negative for neural markers (see Figure S1 in Matsuda et al., 2019).
Cell子刊：蒋辉团队揭示线粒体疾病的代谢补偿机制及治疗靶点|线 …
2021年7月19日 · 呼吸链复合物I亚基由核DNA和mtDNA共同编码，mtDNA编码的复合物I亚基ND1、ND2、ND3、ND4、ND4L、ND5、ND6都会发生突变导致Leber's Heredity Optic Neuropathy（成人视神经退行性疾病），MELAS，Leigh syndrome，MERRF（儿童神经退行性疾病）等恶性疾病。. 众所周知，线粒体是细胞的 ...
Loss of mitochondrial DNA‐encoded protein ND1 results in disruption …
2016年2月29日 · Pathogenic point mutations in the mtDNA gene MT-ND1, which encodes ND1, can result in either isolated symptoms or multiple tissue involvement. These MT-ND1 mutations can cause Leber hereditary optic neuropathy, a form of blindness which presents in midlife as acute or subacute central vision loss due to the specific loss of retinal ganglion ...
The master energy homeostasis regulator PGC-1α exhibits an mRNA …
2023年9月7日 · In this study, we characterised the RNA-binding function of the human master energy homeostasis regulator PGC-1α, showing that its RS domain regulates the mRNA nuclear export pathway and exhibits...
ND1 NADH dehydrogenase subunit 1 [ (human)] - National Center …
2024年10月10日 · Mutation m.3395A > G in MT-ND1 leads to variable pathologic manifestations. Diagnostic value of circulating cell-free mtDNA in patients with suspected thyroid cancer: ND4/ND1 ratio as a new potential plasma marker. Codon optimization is an essential parameter for the efficient allotopic expression of mtDNA genes.