Pulsed-field gel electrophoresis - Wikipedia
Pulsed-field gel electrophoresis (PFGE) is a technique used for the separation of large DNA molecules by applying an electric field that periodically changes direction to a gel matrix. [1] [2] Unlike standard agarose gel electrophoresis, which can separate DNA fragments of up to 50 kb, PFGE resolves fragments up to 10 Mb. [1]
Pulsed-field gel electrophoresis - Nature Protocols
2007年3月29日 · This protocol describes pulsed-field gel electrophoresis (PFGE), a method developed for separation of large DNA molecules. Whereas standard DNA gel electrophoresis commonly resolves...
Pulsed Field Gel Electrophoresis (PFGE) - Microbe Notes
2022年8月23日 · Pulsed Field Gel Electrophoresis (PFGE) is a technique used for the separation of large deoxyribonucleic acid (DNA) molecules by applying to a gel matrix an electric field that periodically changes direction.
What is Pulsed Field Gel Electrophoresis? - NEB
Pulsed field gel electrophoresis, or PFGE, is a technique for separating and sizing large DNA molecules. This animation demonstrates the basic principles of PFGE.
Pulsed-Field Gel Electrophoresis (PFGE): Steps, Applications
Pulsed-field gel electrophoresis (PFGE) is a powerful molecular typing technique by which genomic DNA is isolated from the organism of interest, followed by restriction enzyme analysis.
Pulsed-field gel electrophoresis (PFGE): A review of the "gold ... - PubMed
Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for bacteria typing. The method involves enzyme restriction of bacteria DNA, separation of the restricted DNA bands using a pulsed-field electrophoresis chamber, followed by …
Pulsed Field Gel Electrophoresis - Bio-Rad
PFGE resolves DNA by alternating the electrical field between spatially distinct pairs of electrodes. This technique results in the separation of DNA fragments of up to ~10 Mb by their reorientation and movement at different speeds through the pores of an agarose gel.
Pulsed-field gel electrophoresis (PFGE)-Introduction, Principle, Test
2023年10月4日 · Pulsed-field gel electrophoresis (PFGE) is a powerful laboratory technique used in molecular biology and genetics to separate and analyze large DNA molecules, such as genomic DNA, by their size.
Pulsed Field Gel Electrophoresis: Past, present, and future
2019年5月15日 · PFGE resolves chromosomal-sized DNA molecules in agarose gels and has found application today in public health surveillance. Bacterial subtyping is the main PFGE application due to the discriminatory power, simplicity and low cost of the technique.
Pulsed Field Gel Electrophoresis – The Basics - Bitesize Bio
2020年1月14日 · They are great for visualizing small DNA fragments up to 10 kb, but what if you want to examine really large pieces of DNA or even whole chromosomes? This is where pulsed field gel electrophoresis (PFGE) comes in!