PLGA nanoparticles co-delivering MDR1 and BCL2 siRNA for
2018年5月14日 · Our siRNA-loaded PLGA nanoparticles for co-delivering MDR1 and BCL2 siRNA provide an efficient combination therapy strategy to overcome the chemoresistance of paclitaxel and cisplatin on the...
Preparation of siRNA-encapsulated PLGA nanoparticles for …
Here we provide methods for preparing PLGA NPs that encapsulate small interfering RNA (siRNA). The siRNA NPs are formulated using a double-emulsion solvent evaporation technique with the addition of a small amount of the cationic polymer, polyethyleneimine, which significantly increases siRNA encapsulation.
Polymeric nanoparticles for siRNA delivery and gene silencing
2009年2月9日 · In the present study, we investigated nanoparticles formulated using the biodegradable polymer, poly(d, l-lactide-co-glycolide) (PLGA) for siRNA delivery. A cationic polymer, polyethylenimine (PEI), was incorporated in the PLGA matrix to improve siRNA encapsulation in PLGA nanoparticles.
Inhaled siRNA nanoparticles targeting - Science
2022年6月22日 · Here, we developed a unique lipid-polymer NP platform consisting of lipid-like compound G0-C14 and PLGA-PEG to address the barriers associated with aerosol delivery of siRNA against IL11, a point of signaling convergence downstream of multiple profibrotic stimuli.
PLGA microspheres encapsulating siRNA - PubMed
This specific protocol has been developed to encapsulate a siRNA anti-TNFα in PLGA microspheres, and it has been designed and optimized to achieve high siRNA encapsulation efficiency and slow siRNA release in vitro. However, it can be extended also to other siRNA as well as other RNA or DNA-based oligonucleotides (miRNA, antisense, decoy, etc.).
Preparation of siRNA–PLGA/Fabʹ–PLGA mixed micellar system …
2021年8月18日 · This study aimed to prepare siRNA–PLGA/Fabʹ–PLGA mixed micelles for siRNA delivery to target floating cells and evaluate its formulation in vitro.
High loading efficiency and sustained release of siRNA …
2011年1月1日 · Addition of Ac-BSA increased the encapsulation efficiency at low PLGA concentrations. The PLGA matrix protected siRNA against nuclease degradation, provided a burst release of surface-localized siRNA followed by a triphasic sustained release for two months.
Therapeutic potential of functionalized siRNA nanoparticles …
2019年11月1日 · In the present study, an effective, safe and biocompatible survivin siRNA encapsulated, GalNAc decorated PEGylated PLGA nanoconjugates (NCs) viz., GalNAc@PEG@ siRNA -PLGA were engineered...
Synthesis of PLGA-Lipid Hybrid Nanoparticles for siRNA
Here we describe a versatile platform for siRNA delivery based on PLGA-PEG-cationic lipid nanoparticles by using the double emulsion method. The resulting nanoparticles show high encapsulation efficiency for siRNA (up to 90%) and demonstrate effective downregulation of the target genes in vitro and vivo.
Acid-Sensitive Sheddable PEGylated PLGA Nanoparticles Increase the ...
2016年7月19日 · In the present paper, we report the preparation of novel TNF-α-siRNA-encapsulated PLGA nanoparticles by the standard double emulsion method, but using a unique acid-sensitive surface active agent, i.e., the stearoyl-hydrazone-polyethylene glycol 2000 (PHC) previously synthesized in our laboratory by conjugating polyethylene glycol 2000 ...
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