Optimization of stimulation and staining conditions for intracellular ...
2021年1月1日 · We investigated ideal stimulation and staining conditions for cytokine producing T cells and monocytes in whole blood. PMA + Ionomycin was the ideal stimulant for cytokine-producing T cells, LPS was the best for cytokine-producing monocytes.
Optimal Method to Stimulate Cytokine Production and Its Use in ...
PMA/ionomycin was the optimal stimulant in our study, and could significantly increase production of five cytokines in 6 h without any significant damage to immune cells. PMA activates protein kinase C, while ionomycin is a calcium ionophore, and stimulation with these compounds bypasses the T cell membrane receptor complex and will lead to ...
The Effects of Stimulation with PMA/Ionomycin on CD4+ T Cell
CD4+ T cell frequency and CD4 MFI (mean fluorescence intensity) were evaluated using flow cytometry before and after stimulation with PMA/ION. Results: The frequencies of CD4+ T cells were significantly lower in patients with LRBA deficiency than in HCs before and after treatment.
Molecular pathway profiling of T lymphocyte signal transduction ...
Translation towards a primary, whole blood setting and purified human CD4+ T cells revealed that PMA/CD3 stimulation induced a more pronounced Th1 specific, Lck and PKCθ dependent IFNγ production, whereas PMA/CD28 induced Th2 specific IL …
Concomitant evaluation of PMA+ionomycin‐induced kinase …
2014年1月24日 · We examined intracellular ERK1/2 phosphorylation and IFN-γ production by CD4+ and CD8+ T cells upon polyclonal stimulation with PMA and ionomycin, while monitoring expression of the cytolytic molecule perforin and the T cell activation marker CD38.
Concomitant evaluation of PMA+ionomycin-induced kinase
We examined intracellular ERK1/2 phosphorylation and IFN-γ production by CD4+ and CD8+ T cells upon polyclonal stimulation with PMA and ionomycin, while monitoring expression of the cytolytic molecule perforin and the T cell activation marker CD38.
Single-cell RNA sequencing identifies distinct transcriptomic ...
Immunologists have activated T cells in vitro using various stimulation methods, including phorbol myristate acetate (PMA)/ionomycin and αCD3/αCD28 agonistic antibodies. PMA stimulates protein kinase C, activating nuclear factor-κB, and ionomycin increases intracellular calcium levels, resulting in …
A new simple whole blood flow cytometry-based method for
2004年8月16日 · After stimulation for 3 h with PMA plus ionomycin, cellular responses were associated with surface TNF α expression on the majority of CD3 + T cells and secretion of Th1-associated...
PMA Ionomycin in T-Cell Activation and Calcium Signaling …
The interaction of PMA and ionomycin with T-cells initiates a cascade of intracellular events. PMA, by activating protein kinase C, influences signaling pathways that are crucial for gene transcription relevant to T-cell activation.
PMA and ionomycin treatment decouple the IKK/MAPK and …
In this study, using the calcium ionophore ionomycin and/or PMA on Jurkat T cells, we show that the gene expression program associated with activation of TCR signaling is closely related to...
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