嘌呤霉素（puromycin）抗体检测蛋白合成和翻译抑制的原理和方法
2024年2月10日 · 嘌呤霉素（puromycin）和氨酰-tRNA分子3'末端的结构类似，能够与核糖体的A位点结合，从而被添加到新合成的肽链中去。 puromycin和核糖体的 A位点结合然后掺入延伸的肽链之后，则无法继续参与随后的肽链延伸反应，导致蛋白质合成的提前终止，然后释放出 C-末端 ...
Puromycin - Wikipedia
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, [1] that causes premature chain termination during translation taking place in the ribosome. Part of the molecule resembles the 3' end of the aminoacylated tRNA.
Effect of puromycin and actinomycin D on a persistent mumps …
Puromycin and actinomycin D were used to treat a line of human conjunctiva cells persistently infected with mumps virus (C-M cells) in order to determine where virus synthesis is inhibited. Although 90% of the cells in C-M cultures are infected, little or no infectious virus is produced by most cells in a growing culture.
The effects of actinomycin D and puromycin on the formation of ...
Assuming that the inhibition of ribosomal labeling by puromycin and actinomycin D are due to their known abilities to inhibit protein synthesis and DNA-dependent RNA synthesis, respectively, the present data could be explained by postulating the existence of a ribonucleoprotein intermediate consisting of heavy RNA and some new protein.
Actinomycin and Puromycin: Effects on Sequential Gene
Inhibition of protein synthesis does not influence the induction of those puffs which appear shortly after injection of ecdysone. Puffs which develop after a longer period are delayed in appearance. It is concluded that early reacting genes are involved in those processes leading to the sequential activation of the puffs which appear later.
Differential Effects of Actinomycin D and Puromycin on the …
THE induction of interferon production with synthetic double stranded RNA 1 is suppressed by actinomycin D2,3.
ACTINOMYCIN AND PUROMYCIN: EFFECTS ON SEQUENTIAL GENE …
Inhibition of protein synthesis does not influence the induction of those puffs which appear shortly after injection of ecdysone. Puffs which develop after a longer period are delayed in appearance. It is concluded that early reacting genes are involved in those processes leading to the sequential activation of the puffs which appear later.
The science of puromycin: From studies of ribosome function to ...
2020年1月1日 · Puromycin is produced by Streptomyces alboniger, a gram-positive actinomycete, through a series of enzymatic reactions starting from adenosine triphosphate (ATP) [1]. Structurally, puromycin resembles the 3′ end of aminoacylated tRNA (aa-tRNA), with a modified adenosine base covalently linked to a tyrosine amino acid [2].
Puromycin - an overview | ScienceDirect Topics
Puromycin is produced by Streptomyces alboniger, a gram-positive actinomycete, through a series of enzymatic reactions starting from adenosine triphosphate (ATP) [1]. Structurally, puromycin resembles the 3′ end of aminoacylated tRNA (aa-tRNA), with a modified adenosine base covalently linked to a tyrosine amino acid [2].
Effects of puromycin, acetoxycycloheximide and actinomycin D
Effects of puromycin, acetoxycycloheximide and actinomycin D on protein synthesis in goldfish brain J Neurochem . 1966 Oct;13(10):889-96. doi: 10.1111/j.1471-4159.1966.tb10284.x.