Bile Solubility Test- Principle, Reagents, Procedure and Result ...
2022年8月10日 · Streptococcus pneumoniae is bile soluble whereas all other alpha-hemolytic streptococci are bile resistant. S. pneumoniae has an autolytic enzyme which can be demonstrated by allowing a broth culture to age in the incubator; at 24 hours the broth is turbid; after a few days the medium will become clear.
Bile Solubility Test: Principle, Procedure, Results
2013年5月17日 · Streptococcus pneumoniae is bile soluble, whereas all other alpha-hemolytic streptococci are bile resistant. Sodium deoxycholate (2% in water) will lyse the pneumococcal cell wall. Autolysin of S. pneumoniae is responsible for central depression or umbilication characteristics of older pneumococcal colonies on agar media.
Biochemical Test and Identification of Streptococcus pneumoniae
2022年8月9日 · Biochemical Test and Identification of Streptococcus pneumoniae. They are gram +ve, catalase -ve, oxidase -ve, non-sporing, diplococci bacteria.
Bile Solubility Test- Principle, Procedure, Types, Results, Uses
2022年1月6日 · The bile solubility test is used to identify and distinguish S. pneumoniae from alpha-hemolytic Streptococcus spp. The test may be performed using a cell suspension on a slide or in a tube or by adding the reagent directly to the colony.
Optochin Susceptibility Test for the identification of …
2022年8月10日 · Differentiation of Streptococcus pneumoniae from other viridans streptococci depends on demonstrating optochin susceptibility, bile solubility, reaction with a specific DNA probe, or detection of species-specific capsular polysaccharides. Most clinical microbiology laboratories today depend on the optochin susceptibility test.
Bile Solubility Test: Principles, Methods, and Clinical Uses
2024年7月30日 · Bile solubility testing leverages specific biochemical properties unique to S. pneumoniae, enabling precise identification through relatively simple procedures. The bile solubility test hinges on the unique ability of certain bacterial cell walls to be lysed by bile salts.
3.17.6 Bile Solubility Test | 3.17 Biochemical Tests for the ...
2023年5月9日 · The bile solubility test is used to differentiate Streptococcus pneumoniae from other alpha‐hemolytic Streptococcus spp. It may be performed in a test tube or by direct application of the reagent directly onto…
Bile Solubility Test - Principle, Procedure, Results, & Limitations
2019年1月27日 · The bile solubility test is a microbiological test used to differentiate Streptococcus pneumoniae from other alpha-hemolytic streptococci. S. pneumoniae is bile soluble, while other alpha-hemolytic streptococci are bile resistant.
Bile Solubility Test- Principle, Procedure, Types, Results
Bile solubility test is a biochemical experiment used to distinguish Streptococcus pneumoniae from other alpha-hemolytic streptococci. It is based on the idea that bile salts (e.g., sodium deoxycholate) lyse S. pneumoniae cells by activating an autolytic enzyme, while other alpha-hemolytic streptococci are unaffected and remain intact.
Bile solubility test: Principle, Procedure, and Result - Notes for …
Bile solubility test is based on the unique ability of Streptococcus pneumoniae to lyse in the presence of bile salts (typically sodium deoxycholate). This lysis occurs due to the activation of the autolysin enzyme present in S. pneumoniae, which leads to …