Addgene: Virus Protocol - Generating Stable Cell Lines
This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. Unlike the short-term protein expression observed using transient transfection approaches, generating cell lines using lentiviral vectors enables long-term protein expression studies.
The generation of stably-transfected cell lines is essential for a wide range of applications, such as gene function studies (Grimm, 2004), drug dis- covery assays or the production of recombinant proteins (Wurm, 2004).
Stable Transfection | Thermo Fisher Scientific - US
Explore stable transfection for the introduction of DNA into cells long-term, including selection markers and antibiotics for stable cell line generation.
Creating Stable Cell Lines: Step-by-Step Protocol, Applications, …
2023年8月21日 · Stable cell lines play a crucial role in advancing scientific research, enabling prolonged and consistent gene expression for various applications. This article provides a comprehensive guide for researchers seeking to establish stable cell lines that constitutively express GFP-tagged proteins.
Generation of Stable Expression Mammalian Cell Lines Using …
Lentiviruses are used very widely to generate stable expression mammalian cell lines. They are used for both gene down-regulation (by using shRNA) or for gene up-regulation (by using ORF of gene of interest).
Stable Cell Line Generation - Danaher Life Sciences
Stable cell lines are cellular models that harbor genetic modifications to express specific proteins or biomolecules of interest. Generating stable cell lines involves a sequential series of events intended to produce a uniform population of cells that homogeneously express the transgene.
Generating Mammalian Stable Cell Lines by Electroporation
If the expressed protein needs to be made frequently, it can be best to generate a stable cell line instead of performing repeated transient transfections into mammalian cells. Here, we describe a method to generate stable cell lines via electroporation followed by selection steps.
Lentiviral transduction of mammalian cells for fast, scalable ... - Nature
2016年3月21日 · Lentiviral transduction is an efficient method for the delivery of transgenes to mammalian cells and unifies the ease of use and speed of transient transfection with the robust expression of...
How to generate stable cell lines? - ProteoGenix US
2024年8月23日 · Learn the essentials of stable cell line generation, from vector design to clone selection, and enhance your biomanufacturing success with expert tips and strategies
Stable Cell Lines
Scientists use stable cell lines for diverse applications, ranging from studying cell differentiation, gene expression, cellular events, toxicity, and production of recombinant antibodies and proteins. Stable cell lines are also helpful in the evaluation of drugs in specific environments.
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