T Cell Proliferation - an overview | ScienceDirect Topics
T cell proliferation in response to stimulation by a peptide–MHC complex can be measured by (1) the incorporation of the radioactive nucleotide tritiated thymidine into the DNA of dividing but not resting cells or (2) the dilution of a fluorescent dye, carboxyfluorescein succinimidyl ester (CFSE), that decreases in fluorescence intensity by ...
A genome-scale screen for synthetic drivers of T cell proliferation
2022年3月16日 · Here we identify positive regulators of T cell functions through overexpression of around 12,000 barcoded human open reading frames (ORFs). The top-ranked genes increased the proliferation...
Nature丨基因组筛选T细胞增殖驱动因子 - 知乎 - 知乎专栏
2022年3月29日 · 本研究通过将带条形码的人类开放阅读框 (ORF) 进行过表达处理来识别 T 细胞功能的正调节因子；由此开发了单细胞基因组学方法 OverCITE-seq，用于高通量定量 ORF工程 T 细胞中的转录组和表面抗原；发现了当在 T 细胞中过表达时，LTBR 诱导了深刻的转录和表观基因组重塑，通过经典 NF-κB 通路的组成性激活，导致 T 细胞效应功能和对慢性刺激环境中的衰竭抵抗力增加；s研究结果提供了几种通过诱导合成细胞程序来改进下一代 T 细胞疗法的策略。 使用 …
Reporting T cell proliferation - Nature Methods
2022年5月11日 · To investigate the dynamics of the CD8 + T cell compartment, a team at the Netherlands Cancer Institute led by Ton Schumacher has developed a genetic tracing approach called DivisionRecorder...
T Cell Proliferation - an overview | ScienceDirect Topics
T cell proliferation is a surrogate of T cell function because T cells need to be able to replicate to mount an effective immune response. Mitogens use the intrinsic mechanisms of the T cell to cause proliferation independent of T cell specificity.
A guide to cancer immunotherapy: from T cell basic science to …
2020年5月20日 · Activated T cells produce and consume proliferative/survival cytokines, for example, IL-2, IL-4 and IL-7, and begin to expand in number. If CD4 + CD25 + regulatory T (T reg) cells are...
T-cell metabolism governing activation, proliferation and ...
Dysregulated T-cell metabolism is associated with impaired immunity in chronic infection and cancer and conversely with excessive T-cell activity in autoimmune and inflammatory pathologies. Here we review the key aspects of T-cell metabolism relevant to their immune function, and discuss evidence for the potential to therapeutically modulate T ...
Current estimates of T cell kinetics in humans - ScienceDirect
2019年12月1日 · A study using the most recent definition (CD45RO ― CD27 bright CCR7 + CD95 ―) finds that naïve CD8 + T cells have a proliferation rate of 0.0005 d −1 (doubling time = 1400 days) [18].
CD57 + Memory T Cells Proliferate In Vivo - Cell Press
In this study, we used deuterium labeling to quantify the proliferation of CD57 − and CD57 + memory T cells in vivo and supplemented these analyses with ex vivo measurements of telomere length, telomerase activity, and intracellular expression of the cell-cycle marker Ki67.
Lactate Limits T Cell Proliferation via the NAD(H) Redox State
2020年12月15日 · Quinn et al. report that lactate has an acidity-independent suppressive effect on effector T cell proliferation mediated through a shift from NAD+ to NADH (lactate-induced reductive stress). This impairs glycolysis and glucose-derived serine production, which is required for effector T cell proliferation.