In addition, standard DNA repair mechanisms must be suppressed or modified at telomeres to prevent their being recognized and processed as DNA double-strand breaks. Here, we discuss the players and processes that maintain and regenerate telomere structure.

2022年7月5日 · In this Review, we discuss the mechanistic differences between telomere maintenance by telomerase and ALT, the current methods used to detect each mechanism, the utility of these tests for...

To protect genomic stability, mammalian cells have evolved highly conserved DNA repair mechanisms to remove and repair DNA lesions. Telomeres are composed of long tandem TTAGGG repeats located at the ends of chromosomes. Maintenance of functional telomeres is critical for preventing genome instability.

1995年1月1日 · We find that ultraviolet light-induced pyrimidine dimers in telomeric DNA are repaired less efficiently than endogenous genes but more efficiently than inactive, noncoding regions. We have also measured telomeric length, telomeric DNA damage, and its repair in relation to the progression of aging.

2024年1月2日 · RAD52 functions in telomere repair by recruiting POLD3, a crucial protein in break-induced DNA replication (BIR). The ROS-induced telomeric R-loop also facilitates repair of telomeric DSBs via the CSB-RAD52-POLD3-mediated BIR pathway so that telomeres can be safeguarded from ROS-derived damages .

The telomeric sequence possesses unique features that predispose telomeres to a variety of DNA damage induced by environmental genotoxins. This review briefly describes the relevance of excision repair pathways in telomere maintenance, with the focus on base excision repair (BER), nucleotide excision repair (NER), and mismatch repair (MMR).

4 天之前 · The CHAMP1 complex is also required for heterochromatin assembly and DSB repair in highly-specialized chromosomal regions, such as the highly-compacted telomeres of ALT (Alternative Lengthening of ...

4 天之前 · DNA repair activity of reverse transcriptase may negatively affect prime editing precision. ... White arrows indicate telomeres (small dots), representative of observations from 20 cells. Scale ...

2013年6月1日 · Although telomeres in different organisms (or even in the same organism under different conditions) are maintained by different mechanisms, the disparate processes have the common goals of repairing defects caused by semiconservative replication through G-rich DNA, countering the shortening caused by incomplete replication, and postreplication ...

Studies by us and others have shown the association between the DNA repair factors and telomere function in mammalian cells. Mice deficient in a DNA-break sensing molecule, PARP-1 (poly (ADP)-ribopolymerase), have increased levels of chromosomal instability associated with extensive telomere shortening.