Glycan engineering reveals interrelated effects of terminal galactose ...
Here, we used a new strategy for glycan enrichment and remodeling to study the impact of terminal galactose on ADCC activity for therapeutic IgG1s. Our results indicate that the degree of influence of terminal galactose on in vitro ADCC activity depends on the presence or absence of the core fucose, which is typically linked to the first N ...
Fc galactosylation follows consecutive reaction kinetics and …
2021年3月8日 · In this study, we investigated the kinetics of terminal galactose formation during CHO cell culture process, galactosylation-mediated protein conformation, and CDC potency variation to understand the structural, functional and mechanistic aspect of …
Galectin-3 N-terminal tail prolines modulate cell activity and ... - PNAS
2021年5月5日 · The functional role of the N-terminal tail (NT) of galectin-3 (Gal-3) with its numerous proline residues (27 out of 113 in human Gal-3) has remained enigmatic since its discovery. Our study enlightens the galectin field on the role of specific Gal-3 NT prolines and opens a window into understanding the mechanisms of Gal-3 proline polymorphisms ...
Fc-galactosylation modulates antibody-dependent cellular …
2016年5月1日 · We enzymatically modulate the galactose levels of four different batches of mAb1 and confirm that the presence of terminal galactose enhances ADCC activity. The ADCC enhancing effect of galactose is observed in 4 additional monoclonal antibodies derived using standard CHO-based manufacturing processes.
Glycosylation: mechanisms, biological functions and clinical ...
There is a common core structure for all N-glycans (asn-GlcNAc2Man3), which is even further extended by terminal glycan residues. 13 These glycan residues, which mainly include GlcNAc, mannose, galactose, fucose and sialic acid (N-acetylneuraminic acid/Neu5Ac), could significantly affect the structure of N-glycan.
Liver-Targeted Delivery of Oligonucleotides with N …
2021年6月14日 · GalNAc-based delivery relies on the fact that liver hepatocytes abundantly and specifically express the asialoglycoprotein receptor that binds and uptakes circulating glycoproteins via receptor-mediated endocytosis. In recent years, encouraging progress has been made in the field of GalNAc conjugates.
Galactosylation - an overview | ScienceDirect Topics
In mammalian cells, the terminal GlcNAc residues are usually modified with galactose residues by the action of β4GalT. The transfer of galactose requires a terminal GlcNAc-containing glycoprotein, UDP-galactose, and β4GalT.
Enhanced Galactosylation of Monoclonal Antibodies: Using …
2018年3月23日 · Galactosyltransferase from porcine mesenteric lymph node (EC 4.2.1.38) added galactose to the terminal GlcNAc of one arm on glycopeptides — the more mobile α1,6 arm — and slowed incorporation of galactose on the α1,3 arm (29). Thus, there is more G1F(1→6) glycan than G1F(1→3).
Novel lamprey antibody recognizes terminal sulfated galactose epitopes ...
2021年6月3日 · The terminal galactose residues of N- and O-glycans in animal glycoproteins are often sialylated and/or fucosylated, but sulfation, such as 3-O-sulfated galactose (3-O-SGal), represents an ...
The glycosylation design space for recombinant lysosomal …
2019年4月30日 · The AMR expressed primarily on liver hepatocytes recognizes glycoproteins with uncapped terminal galactose (Gal) or N-acetyl-galactosamine (GalNAc) residues, and mediates clearance from the ...