Ligation (molecular biology) - Wikipedia
Ligation occurs naturally as part of numerous cellular processes, including DNA replication, transcription, splicing, and recombination, and is also an essential laboratory procedure in …
DNA Ligation Overview: Mechanism, Types, Applications & Trends ...
DNA ligation is the process of joining two strands or fragments of DNA molecules through a phosphodiester bond formed by the enzyme DNA ligase. DNA ligase is involved in maintaining …
连接反应 - 百度百科
连接反应Ligation : 在双螺旋DNA单链上，连接缺口处两个相邻碱基形成磷酸二脂键（也可用于连接RNA 平末端连接）。
Addgene: Protocol - How to Ligate Plasmid DNA
This reaction, called ligation, is performed by the T4 DNA ligase enzyme. The DNA ligase catalyzes the formation of covalent phosphodiester linkages, which permanently join the …
DNA Ligation | NEB
Ligation of DNA is a critical step in many modern molecular biology workflows. The sealing of nicks between adjacent residues of a single-strand break on a double-strand substrate and the …
Ligation and Ligases - CSH Protocols
Ligation of DNA is a three-step reaction: (i) formation of a covalent ligase-AMP intermediate, (ii) transfer of the AMP to the 5′-phosphoryl terminus of DNA, and (iii) attack on the AMP–DNA …
Nature-inspired protein ligation and its applications
2023年2月21日 · Protein ligation, in which two or more polypeptides are covalently linked, is a powerful strategy for generating semisynthetic products and for controlling polypeptide topology.
LIGATION中文(简体)翻译：剑桥词典 - Cambridge Dictionary
ligation翻译：结扎。了解更多。 示例中的观点不代表剑桥词典编辑、剑桥大学出版社和其许可证颁发者的观点。
Module 1.3: DNA Ligation and Bacterial Transformation
You will transform today’s ligation reactions into bacteria. During ’transformation,’ a single plasmid from the ligation mixture enters a single bacterium and, once inside, replicates and expresses …
DNA Ligation - NEB
DNA Ligation is the formation of a covalent bond between adjacent DNA fragments, frequently a vector and a gene of interest. Ligation is usually the final step before transformation in the …