mCherry :: Fluorescent Protein Database
2022年8月18日 · mCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer with low acid sensitivity.
mCherry Sequence and Map - SnapGene
Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. BsrGI is typically used at 37°C, but is even more active at 60°C. Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be …
Decoding mCherry: Advancements in Fluorescent Proteins
What distinguishes mCherry from other proteins is its optimal balance of photostability, brightness, and excellent pH resistance, making it the most widely used red fluorescent protein in biomedical research. mCherry fluorescence is a constitutive and inherent characteristic of this protein.
Engineering of mCherry variants with long Stokes shift, red …
MCherry, the Discosoma sp. mushroom coral-derived monomeric red fluorescent protein (RFP), is a commonly used genetically encoded fluorophore for live cell fluorescence imaging.
mCherry contains a fluorescent protein isoform that interferes …
The short mCherry isoform creates in average a 19% background fluorescence compared with the original sequence. The control shows the background fluorescence of the S. cerevisiae uracil-deficient strain. Each measurement was performed in biological triplicates.
Construction of an Integrated mCherry Red Fluorescent Protein ...
In this study, we aimed to construct a stable RFP mCherry expression system, whose encoding gene was integrated into the bacterial chromosome via double-crossed homologous recombination, and use it for labeling WCFS1 with the goal of avoiding the potential loss of non-chromosomal plasmids along with intestinal growth.
MCherry Fluorescent Protein - Proteopedia, life in 3D
The official biological process of mCherry is bioluminescence and this process allows mCherry to aid in the generation of precursor metabolites and gene expression analytics. mCherry is most commonly used as a fluorescent reporter, acting as a labeled tag …
Fluorescence Fluctuation Spectroscopy of mCherry in Living Cells
The red fluorescent protein mCherry is of considerable interest for fluorescence fluctuation spectroscopy (FFS), because the wide separation in color between mCherry and green fluorescent protein provides excellent conditions for identifying protein interactions inside cells.
Photoactivatable mCherry for high-resolution two-color ... - Nature
2009年1月25日 · Here we report several irreversibly photoactivatable derivatives of mCherry 17, named PAmCherry proteins, with excellent photoactivation contrast over background, advanced photostability and high...
Super-resolution imaging with mCherry | Nature Methods
2017年8月1日 · Exploiting this phenomenon, they were able to stochastically activate mCherry molecules and generate super-resolution images of mCherry-labeled tubulin, clathrin, and other proteins.
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