pET-32a(+) Sequence and Map - SnapGene
Bacterial vector for expressing thioredoxin fusion proteins with an enterokinase site. For other reading frames, use pET-32b (+) or pET-32c (+). Sequence Author: MilliporeSigma (Novagen) …
pET-32a (+) vector map and sequence
General Plasmid Transform Protocol. 1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do …
69015 Sigma-Aldrich pET-32a(+) DNA - Novagen - EMD Millipore
The pET-32a (+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein (1). Cloning sites are available for …
The maps for pET-32b(+) and pET-32c(+) are the same as pET-32a(+) (shown) with the following exceptions: pET-32b(+) is a 5899bp plasmid; subtract 1bp from each site beyond BamH I at …
pET-32a(+)-NRG1B1 - Addgene
Plasmid pET-32a (+)-NRG1B1 from Dr. Paul Burridge's lab contains the insert NRG1-beta 1 and is published in Stem Cell Reports. 2020 Jan 9. pii: S2213-6711 (19)30446-1. doi: …
Recombinant Expressed Vector pET32a (+) S Constructed by …
In this work, we report the development of an effective, inexpensive method named the LIC method to construct the expression vector pET32a (+) S and pET32a (+) S-phoN2, which can …
Vector: pET-32a(+) | Genophore
The pET-32a(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein . Cloning sites are available for producing …
69015 Sigma-Aldrich pET-32a(+) DNA - Novagen - Merck
pET-32a (+) DNA - Novagen Novagen's pET-32a (+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein. - Find …
(PDF) Construction of pET-32 α (+) Vector for Protein
2012年12月1日 · The procedures include that acquisition of the exogenous DNA fragment for construction of the vector, subcloning the DNA fragment into pET-32 α (+) expression vector, …
pET-32a (+) DNA - EcoliWiki
pET-32a (+) is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein [1]. You can help EcoliWiki by writing about the …