By deciphering lariat RNA sequences, we identified an AG-rich (RAAAAVAAAR) motif and a UC-rich (UCUCUYUCUC) motif for pollen-specific and the other three tissues-retained lariat RNAs, respectively. Reconstitution of the pollen-specific AG-rich motif indeed enhanced lariat RNA retention in plants.

Here, we report the first structures of Dbr1 alone and in complex with several synthetic RNA compounds that mimic the branchpoint in lariat RNA. The structures, together with functional data on Dbr1 variants, reveal the molecular basis for 2′,5′-phosphodiester recognition and explain why the enzyme lacks activity toward 3′,5 ...

We describe a general and efficient two-step strategy for lariat RNA synthesis. In the first step, a deoxyribozyme synthesizes 2′,5′-branched RNA. In the second step, T4 RNA ligase closes the loop that completes the lariat.

2012年6月14日 · Lariats are discarded byproducts of RNA splicing, the process by which genetic instructions for making proteins are assembled. A new study has found hundreds more lariats than ever before, yielding new information about how splicing occurs and how it …

Unlike RNA from α-amanitin–treated cultured cells, which is enriched for metabolically stable molecules, total RNA derived from whole tissues is a mixture of short lived and more stable molecules. We analyzed intronic RNAs derived from short introns (<1,500 nt) and determined the branchpoint usage.

The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5′ end of the intervening sequence joined to a site near the 3′ splice site. This lariat structure, w...

2016年11月21日 · Lariat RNAs were associated with the DCL1/HYL1 dicing complex in vivo and competitively inhibited the binding of HYL1 with pri-miRNA. Consistent with the impacts of lariat RNAs on miRNA biogenesis, over-expression of lariat RNAs reduced miRNA accumulation.

Here, we describe a large-scale analysis to systematically identify the lariat RNAs (i.e., intronic circular RNAs) in Arabidopsis by utilizing the RNA-sequencing data.

These stable lariats bearing a snoRNA (or slb-snoRNAs) can be found in the nucleus and cytoplasm associated with snoRNA binding proteins. They do not function as modification guide RNAs and their export competes with snoRNA maturation. Therefore, slb-snoRNAs provide a new level of regulation of snoRNAs and snoRNA binding proteins.

2014年9月24日 · The spliceosome enzyme complex removes intron sequences from RNA transcripts to form messenger RNA. The crystal structure of a lasso-shaped RNA suggests a mechanism for this splicing process.