Adeno-associated Virus (AAV) Guide - Addgene
Eleven serotypes of AAV have thus far been identified, with the best characterized and most commonly used being AAV2. These serotypes differ in their tropism, or the types of cells they infect, making AAV a very useful system for preferentially transducing specific cell types.
Adeno-associated virus as a delivery vector for gene therapy of …
2024年4月3日 · Distinct serotypes of wtAAV are characterized by variations of VPs and ITRs and have been isolated primarily from humans and non-human primates (NHPs). 12,13,25 Human seroprevalence studies have ...
Engineered adeno-associated virus 3 vector with reduced
2021年4月29日 · By introducing three substitutions (S472A, S587A, and N706A) on the surface loop of AAV3B capsid protein, we generated a triple mutant AAV3 (AAV.GT5) vector with less reactivity to anti-AAV...
Various AAV Serotypes and Their Applications in Gene Therapy: …
2023年3月1日 · In this article, we present a review of AAV discovery, properties, different serotypes, and tropism, and a following detailed explanation of their uses in gene therapy for disease of different organs and systems.
Prevalence of serum IgG and neutralizing factors against adeno ...
Adeno-associated viruses (AAVs) are small, nonenveloped single-stranded DNA viruses that require helper viruses to facilitate efficient replication. Despite the presence of humoral responses to the wild-type AAV in humans, AAV remains one of the most promising candidates for therapeutic gene transfe …
AAV Serotype Screening System - PackGene Biotech
2024年3月28日 · The TRACER system, abbreviated from Tropism Redirection of AAV by Cell-type-specific Expression of RNA, is a serum type screening method developed for the central nervous system.
AAV Vector Immunogenicity in Humans: A Long Journey to …
Gene therapy with adeno-associated virus (AAV) vectors has demonstrated safety and long-term efficacy in a number of trials across target organs, including eye, liver, skeletal muscle, and the central nervous system.
Seroprevalence of adeno-associated virus types 1, 2, 3, 4, 5
2024年7月10日 · A Seroprevalence of neutralizing antibodies (NAbs) against different adeno-associated virus (AAV) serotypes in 100 healthy human serum samples from residents of the Basque Country. Samples were...
Cellular selectivity of AAV serotypes for gene delivery in neurons …
By use of multiple different AAV-GFP serotypes with CMV/CAG promoters, we demonstrate differences in infectivity and gene expression in primary astrocytes and neurons, as well as tropism in multiple regions of the brain, stability of AAV-GFP expression between 3–6 weeks post ICV injection and expression of AAV-GFP in ventral midbrain areas.
Adeno-Associated Virus Gene Therapy Landscape
AAV serotypes differ in their tropism i.e. their ability to infect certain cell types, principally due to their different cell surface receptors. Our current understanding of inherent AAV tropism is outlined in Table 2.