4-Nitrophenol - Wikipedia
4-Nitrophenol is a product of the enzymatic cleavage of several synthetic substrates such as 4-nitrophenyl phosphate (used as a substrate for alkaline phosphatase), 4-nitrophenyl acetate (for carbonic anhydrase), 4-nitrophenyl-β-D-glucopyranoside and other sugar derivatives which are used to assay various glycosidase enzymes.
An innovative and cost-effective way to estimate alkaline phosphatase ...
2021年2月15日 · Alkaline phosphatase (ALP) assay, which includes the estimation of its activity in serum is a common biochemical test done for the assessment of various pathological conditions [1 - 4]. This assay measures the conversion of para-nitrophenyl phosphate to a yellow coloured compound, i.e. para-nitrophenol (PNP) at pH 10.5 [5].
Kinetic studies with alkaline phosphatase in the presence and …
2006年11月3日 · A very robust and inexpensive kinetic assay for determining rates of hydrolysis of p-nitrophenyl phosphate by the enzyme alkaline phosphatase is presented. The reaction increases in rate with increas...
Alkaline Phosphatase - StatPearls - NCBI Bookshelf
2020年8月20日 · Alkaline phosphatases (ALPs) are a group of isoenzymes located on the outer layer of the cell membrane. They catalyze the hydrolysis of organic phosphate esters found in the extracellular space. Zinc and magnesium are essential cofactors of this enzyme.
Alkaline Phosphatase: An Overview - PMC - National Center for ...
2013年11月26日 · Alkaline phosphatase (ALP; E.C.3.I.3.1.) is an ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values. Alkaline phosphatase is divided into four isozymes depending upon the site of tissue expression that are Intestinal ALP, Placental ALP, Germ cell ALP and tissue nonspecific alkaline ...
Three genes (named ALPI, ALPP, and ALPPL2) encode the tissue-specific intestinal, placental, and germ-cell ALPs, whereas the fourth gene (named ALPL and also known as TNSALP) encodes the tissue non-specific ALP (TNSALP) that is abundantly expressed in the skeleton, liver, kidney, and developing teeth.
The activity of the enzyme alkaline phosphatase can be conveniently measured spectrophotometrically using 4-nitrophenylphos- phate as substrate. This article describes an integrated series of experiments involving the enzyme in which a number of valuable
Alkaline Phosphatase - an overview | ScienceDirect Topics
Alkaline phosphatase is typically measured using 4-nitrophenol phosphate as a substrate and 2-amino-2-methyl-1-propanol (AMP) or diethanolamine (DEA) as a buffer, although DEA yields higher ALP values. Isoenzymes of ALP can be distinguished using electrophoresis, HPLC, and other methods (Evans, 2009).
Alkaline Phosphatase - an overview | ScienceDirect Topics
Alkaline phosphatase is a nonspecific enzyme which hydrolyzes many types of phosphate esters and is present in multiple molecular forms, i.e., isoenzymes. The endogenous substrate of AP is unknown, but the enzyme catalyzes the dephosphorylation of ATP and is thought to be associated with energy-requiring membrane “pumps.”
4-Nitrophenol in 4-nitrophenyl phosphate, a substrate for alkaline ...
1977年12月1日 · We used paired-ion high-performance liquid chromatography to determine the 4-nitrophenol content of 4-nitrophenyl phosphate, a substrate for alkaline phosphatase analysis. This was done on a reversed-phase column with a mobile phase of methanol/water, 45/55 by vol, containing 3 ml of tetrabutylammonium phosphate reagent per 200 ml of solvent ...