Auxotrophy - Wikipedia
In genetics, a strain is said to be auxotrophic if it carries a mutation that renders it unable to synthesize an essential compound. For example, a yeast mutant with an inactivated uracil synthesis pathway gene is a uracil auxotroph (e.g., if the yeast Orotidine 5'-phosphate decarboxylase gene is inactivated, the resultant strain is a uracil ...
Understanding Auxotrophic Mutants: Yeast and Bacterial
Auxotrophic mutants are bacteria, yeast, protoplast or mammalian host cell strains that can’t produce a nutrient vital for growth due to genetic mutations. As a result, these strains are unable to survive in media lacking that specific nutrient unless it is provided externally.
Isolation and Molecular Identification of Auxotrophic Mutants to ...
Auxotrophic markers have been widely used to construct genetic systems in bacteria and eukaryotes and in some archaeal species. Here, we isolated four auxotrophic mutants of Natrinema sp. J7-2, via 1-methyl-3-nitro-1-nitroso-guanidin mutagenesis, and designated them as J7-2-1, J7-2-22, J7-2-26, and J7-2-52, respectively.
Auxotrophic Mutant - an overview | ScienceDirect Topics
An auxotrophic mutant refers to a type of mutant organism that is unable to grow on a minimal medium and requires the addition of specific compounds for growth.
Auxotrophic Mutant - an overview | ScienceDirect Topics
Auxotrophic mutants are generally useful for the production of intermediate metabolite of the straight chain pathway but they can also be useful for the production of branched pathway amino acids such as lysine, threonine and methionine.
The social network of microorganisms — how auxotrophies shape …
A study using amino-acid-auxotrophic yeast strains demonstrated that changes in amino acid availability can modulate the types of interaction between species. Depending on the resource availability, the interaction of these strains changed from obligatory and facultative mutualism to competition and parasitism 69 .
6.3: Auxotrophs and selective media - Biology LibreTexts
Auxotrophs are microorganisms that are unable to synthesize an essential nutrient because of a gene mutation. Many laboratory strains carry multiple mutations that interfere with the synthesis of essential nutrients.
Auxotrophic Mutant - an overview | ScienceDirect Topics
Aspergillus strains were studied for development of multiple gene deletion auxotrophic mutants. Collective mutations in genes intricated in color pigmentation (fwnA and olvA) with selected auxotrophic markers (pyrG, nicB, argB, and adeA) were used by targeted deletion, omitting UV mutagenesis of the strains (Niu et al., 2016).
Isolation of Auxotrophic Mutants of Diploid Industrial Yeast …
2004年4月7日 · Here we show that mutants carrying auxotrophic recessive mutations can be routinely isolated in industrial sake strains by replica plating at frequencies ranging from 5.3 × 10 −4 to 2.0 × 10 −3.
Metabolic adaptation to vitamin auxotrophy by leaf-associated …
2022年8月20日 · Here, we investigated auxotrophies in bacteria isolated from Arabidopsis thaliana leaves and found that up to half of the strains have auxotrophic requirements for biotin, niacin, pantothenate...
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