CRISPR/Cas系统的新星1——Cas12a - 知乎 - 知乎专栏
研究结果表明，基于crispr/cas12系统可高效、快速诊断covid-19。 Cas12核酸酶的这些应用使科学家们能够扩大基因组编辑在各个领域的范围，包括诊断新型病毒。
CRISPR/Cas12a基因组编辑技术及应用 - 知乎 - 知乎专栏
2024年1月3日 · CRISPR/Cas是细菌和古细菌中一种适应性免疫系统，赋予细菌抵抗外源可移动基因元件 (mobilegeneticelement,MGE)入侵的能力。 免疫系统发挥功能由三个步骤完成: (1)适 …
Cas12a - Wikipedia
The acronym CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) refers to the invariant DNA sequences found in bacteria and archaea which encode Cas proteins and their …
Programmable RNA detection with CRISPR-Cas12a - Nature
2023年9月5日 · Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate …
技术 | Cas9或Cas12？如何挑选合适的CRISPR系统 - 知乎
Cas12是CRISPR万神殿的一个较新成员，因其在诊断方面的潜在用途而受到特别关注。 但是，Cas9和Cas12之间的主要区别是什么，以及你如何决定哪个适合你的研究？
CRISPR–Cas12-based detection of SARS-CoV-2 - Nature
2020年4月16日 · We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR–Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab …
A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12…
2022年9月27日 · This review aims to provide detail on two classes (class 1 and 2) of the CRISPR/Cas system, and the mechanisms of all Cas proteins, including Cas12, Cas13, and …
A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12…
Cas variants stimulated genetic research and propelled the CRISPR/Cas tool for manipulating and editing nucleic acid sequences of living cells of diverse organisms. This review aims to provide …
An engineered CRISPR-Cas12a variant and DNA-RNA hybrid …
2021年3月19日 · Here, we present a CRISPR-based diagnostic assay that is robust to viral genome mutations and temperature, produces results fast, can be applied directly on …
Recent application of CRISPR-Cas12 and OMEGA system for
2024年1月3日 · The CRISPR-Cas12 system contains a variety of molecules, ranging from the relatively large Cas12a (1,200–1,500 amino acids [aa]) known as the CRISPR-Cas12 …