A Hitchhiker’s guide to CRISPR editing tools in bacteria:
2024年2月12日 · To fully grasp CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technologies, it is important to understand where they come from. Importantly, these technologies were not invented from scratch but instead derived from bacterial CRISPR-Cas systems, the only known adaptive immune systems in bacteria and archaea (Marraffini, 2015).
CRISPR-cas system: biological function in microbes and its use to …
2019年7月5日 · In this review, we discuss how the newly popular CRISPR-cas system has been applied to combat antibiotic resistance in both extracellular and intracellular pathogens. We also review a recently developed method in which nano-size CRISPR complex was used without any phage to target the mecA gene.
Genome Editing in Bacteria: CRISPR-Cas and Beyond - MDPI
2021年4月15日 · CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is the only known adaptive and hereditary immune response in prokaryotes. It is present in about 50% of bacteria and 90% of archaea [3]. CRISPR-Cas acts by recompiling and storing genetic sequences from invader bacteriophages and noxious plasmids as spacers.
Systematically attenuating DNA targeting enables CRISPR-driven …
2023年2月8日 · Here, we show that systematically attenuating DNA targeting activity enables RecA-mediated repair in different bacteria, allowing chromosomal cleavage to drive genome editing.
Engineered CRISPRa enables programmable eukaryote-like gene
2019年8月26日 · Our CRISPRa device can activate σ 54 -dependent promoters with biotechnology relevance in non-model bacteria. It also supports orthogonal gene regulation on multiple levels. Combining our CRISPRa...
Molecular Mechanisms of CRISPR-Cas Immunity in Bacteria
2020年11月23日 · Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas loci guard their hosts with an adaptive immune system against foreign nucleic acids. Protection starts with an immunization phase, in which short pieces of the invader's genome, known as spacers, are captured and integrated into the CRISPR locus after infection.
A decade of discovery: CRISPR functions and applications
2017年6月5日 · In just a decade, the characterization of CRISPR–Cas systems has established a novel means of adaptive immunity in bacteria and archaea and deepened our understanding of the interplay between...
CRISPR-Cas9: A fascinating journey from bacterial immune system …
2021年1月1日 · Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas system has been discovered as an adaptive-immune system in prokaryotes. Microbes like bacteria and archaea use CRISPR-Cas9 as a part of their defense mechanism to ward off the virus and cleave their DNA.
Programmable CRISPR‐Cas transcriptional activation in bacteria
2020年7月13日 · Here, we present a generalizable platform for screening and selection of functional bacterial CRISPR‐Cas transcription activators. Using this platform, we identified a novel CRISPR activator, dCas9‐AsiA, that could activate gene expression by more than 200‐fold across genomic and plasmid targets with diverse promoters after directed ...
CRISPR-Cas: Converting A Bacterial Defence Mechanism into A …
This review illustrates the mechanisms behind CRISPR-Cas systems as a means of bacterial immunity against phage invasion and how these systems were engineered to originate new genetic manipulation tools. Newfound CRISPR-Cas technologies and the up-and-coming applications of these systems on healthcare and other fields of science are also discussed.