Monitoring CAR T cell generation with a CD8-targeted lentiviral vector ...
2021年12月10日 · LV-exposed human donor peripheral blood mononuclear cells (PBMCs) were evaluated for a panel of 400 immune response-related genes including LV-specific probes. The resulting data revealed a trimodal expression for the CAR and CD8A, demanding a careful distribution-based identification of CAR T cells and CD8+ lymphocytes in scRNA-seq analysis.
Fate induction in CD8 CAR T cells through asymmetric cell division
2024年8月28日 · Here we show that target-induced proximity labelling enables isolation of first-division proximal-daughter and distal-daughter CD8 CARTs that asymmetrically distribute their surface proteome and...
CD8α Structural Domains Enhance GUCY2C CAR-T Cell Efficacy
2024年9月24日 · The consensus within the field has identified three major obstacles that contribute to the inability of CAR-T cells to successfully eradicate solid tumor lesions: (1) trafficking of the CAR-T cells to, and ingress of T cells within, the solid tumor space, (2) the persistence and concomitant functions of CAR-T cells within the suppressive tumor ...
Cytotoxic CD8 + T cells in cancer and cancer immunotherapy
2020年9月15日 · To bypass the isolation and expansion of autologous tumour-reactive CD8 + T cells, the use of bulk T cells from the peripheral blood for CAR T-cell manufacturing has been applied, and has...
Monitoring CAR T cell generation with a CD8-targeted ... - Cell Press
2021年12月10日 · We have set up a nanowell-based scRNA-seq approach for LV-mediated CAR delivery making use of the high selectivity of CD8-LV for CD8+ T cells. In particular, we have performed a targeted gene amplification analysis of untransduced CD8-LV- and VSV-LV-generated CAR T cell products expanded for a short period of time.
Emerging clinical data are proving the potential of CD22 and B cell maturation antigen (BCMA)-directed CAR T cells to eradicate leukemia and multiple myeloma, respectively, heralding a new era for the treatment of blood cancers.10,11.
Generation of T-cell-receptor-negative CD8αβ-positive CAR T …
2022年8月8日 · Delaying CAR expression and calibrating its signalling strength in TiPS enabled the generation of human TCR – CD8αβ + CAR T cells that perform similarly to CD8αβ + CAR T cells from...
Generation of T-cell-receptor-negative CD8αβ-positive CAR T
Delaying CAR expression and calibrating its signalling strength in TiPS enabled the generation of human TCR-CD8αβ + CAR T cells that perform similarly to CD8αβ + CAR T cells from peripheral blood, achieving effective tumour control on systemic administration in a mouse model of leukaemia and without causing graft-versus-host disease.
Monitoring CAR T cell generation with a CD8-targeted ... - PubMed
2021年10月5日 · Quantifying gene expression in individual cells can substantially improve our understanding about complex genetically engineered cell products such as chimeric antigen receptor (CAR) T cells. Here we designed a single-cell RNA sequencing (scRNA-seq) approach to monitor the delivery of a CD19-CAR gen …
Engineered CAR T Cells Targeting the Cancer-Associated Tn …
2016年6月21日 · We developed a CAR that recognized cancer-associated Tn glycoform of MUC1, a neoantigen expressed in a variety of cancers. Anti-Tn-MUC1 CAR T cells demonstrated target-specific cytotoxicity and successfully controlled tumor growth in xenograft models of T cell leukemia and pancreatic cancer.