Cell-free gene expression | Nature Reviews Methods Primers
2021年7月15日 · Cell-free gene expression (CFE) harnesses the transcription and translation machinery of living cells to enable protein synthesis in vitro through the expression of natural or synthetic DNA....
Cell-Free Protein Expression | Thermo Fisher Scientific - US
In vitro protein expression (also known as in vitro translation, cell-free protein expression, cell-free translation, or cell-free protein synthesis) is a technique that enables researchers to rapidly express and manufacture small amounts of functional proteins.
Cell-free gene expression: an expanded repertoire of applications
It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms from non-model organisms and multiplexed strategies for ...
Cell-Free Protein Expression | NEB
Cell-free protein synthesis (CFPS) is a protein expression approach that enables production of a target protein without the use of living cells. In CFPS, a solution containing all the cellular machinery needed to direct protein synthesis (e.g., ribosomes, tRNAs, enzymes, cofactors, amino acids, etc.) is used to transcribe and translate a ...
A rapid cell-free expression and screening platform for antibody ...
2023年7月3日 · Antibody discovery is bottlenecked by the individual expression and evaluation of antigen-specific hits. Here, we address this bottleneck by developing a workflow combining cell-free DNA...
Cell-Free Protein Expression | Thermo Fisher Scientific - US
We have optimized several cell-free expression systems for the rapid synthesis of recombinant proteins, utilizing bacterial, rabbit reticulocyte, and human derived lysate systems. The bacterial systems are ideal for producing high protein yield, while the mammalian-based systems are more likely to produce proteins with native posttranslational ...
Cell-free gene expression: an expanded repertoire of applications …
2019年11月28日 · Cell-free gene expression (CFE) is the activation of transcription and translation using crude cellular extracts instead of intact cells.
Biotechnology Applications of Cell-Free Expression Systems
2021年12月8日 · In this study, new and emerging applications of cell-free platforms, with a specific focus on cell-free protein synthesis (CFPS), will be examined. The current and near-future role of CFPS within metabolic engineering, prototyping, and biomanufacturing will be investigated as well as how the integration of machine learning is beneficial to ...
Cell-Free Gene Expression: Methods and Protocols - Springer
This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high ...
A User’s Guide to Cell-Free Protein Synthesis - MDPI
2024年2月1日 · Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more.